3PMC

Crystal structure of the sporulation inhibitor pXO2-61 from Bacillus anthracis

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3PMC の概要

• エントリーDOI: 10.2210/pdb3pmc/pdb
• 関連するPDBエントリー: 3PMD
• 分子名称: Uncharacterized protein pXO2-61/BXB0075/GBAA_pXO2_0075, IODIDE ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: globin fold, non-heme globin, sporulation, bacillus anthracis, kinase sensor domain, chloride coordination, fatty acid-binding protein, lipid binding protein
• 由来する生物種: Bacillus anthracis (anthrax)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37505.34

構造登録者

Stranzl, G.R.,Santelli, E.,Bankston, L.A.,La Clair, C.,Bobkov, A.,Schwarzenbacher, R.,Godzik, A.,Perego, M.,Grynberg, M.,Liddington, R.C. (登録日: 2010-11-16, 公開日: 2011-01-19, 最終更新日: 2024-11-06)

主引用文献

Stranzl, G.R.,Santelli, E.,Bankston, L.A.,La Clair, C.,Bobkov, A.,Schwarzenbacher, R.,Godzik, A.,Perego, M.,Grynberg, M.,Liddington, R.C.
Structural Insights into Inhibition of Bacillus anthracis Sporulation by a Novel Class of Non-heme Globin Sensor Domains.
J.Biol.Chem., 286:8448-8458, 2011

PubMed Abstract: Pathogenesis by Bacillus anthracis requires coordination between two distinct activities: plasmid-encoded virulence factor expression (which protects vegetative cells from immune surveillance during outgrowth and replication) and chromosomally encoded sporulation (required only during the final stages of infection). Sporulation is regulated by at least five sensor histidine kinases that are activated in response to various environmental cues. One of these kinases, BA2291, harbors a sensor domain that has ∼35% sequence identity with two plasmid proteins, pXO1-118 and pXO2-61. Because overexpression of pXO2-61 (or pXO1-118) inhibits sporulation of B. anthracis in a BA2291-dependent manner, and pXO2-61 expression is strongly up-regulated by the major virulence gene regulator, AtxA, it was suggested that their function is to titrate out an environmental signal that would otherwise promote untimely sporulation. To explore this hypothesis, we determined crystal structures of both plasmid-encoded proteins. We found that they adopt a dimeric globin fold but, most unusually, do not bind heme. Instead, they house a hydrophobic tunnel and hydrophilic chamber that are occupied by fatty acid, which engages a conserved arginine and chloride ion via its carboxyl head group. In vivo, these domains may therefore recognize changes in fatty acid synthesis, chloride ion concentration, and/or pH. Structure-based comparisons with BA2291 suggest that it binds ligand and dimerizes in an analogous fashion, consistent with the titration hypothesis. Analysis of newly sequenced bacterial genomes points to the existence of a much broader family of non-heme, globin-based sensor domains, with related but distinct functionalities, that may have evolved from an ancestral heme-linked globin.

PubMed: 21216948
DOI: 10.1074/jbc.M110.207126

実験手法

X-RAY DIFFRACTION (1.49 Å)