3PCV

Crystal structure analysis of human leukotriene C4 synthase at 1.9 angstrom resolution

3PCV の概要

• エントリーDOI: 10.2210/pdb3pcv/pdb
• 関連するPDBエントリー: 2PNO
• 分子名称: Leukotriene C4 synthase, GLUTATHIONE, DODECYL-BETA-D-MALTOSIDE, ... (7 entities in total)
• 機能のキーワード: membrane protein, helix bundle, homo trimer, mgst, mapeg, lyase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus outer membrane; Multi-pass membrane protein: Q16873
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23776.03

構造登録者

Saino, H.,Ago, H.,Miyano, M. (登録日: 2010-10-22, 公開日: 2011-03-16, 最終更新日: 2023-11-01)

主引用文献

Saino, H.,Ukita, Y.,Ago, H.,Irikura, D.,Nisawa, A.,Ueno, G.,Yamamoto, M.,Kanaoka, Y.,Lam, B.K.,Austen, K.F.,Miyano, M.
The catalytic architecture of leukotriene C4 synthase with two arginine residues
J.Biol.Chem., 286:16392-16401, 2011

PubMed Abstract: Leukotriene (LT) C(4) and its metabolites, LTD(4) and LTE(4), are involved in the pathobiology of bronchial asthma. LTC(4) synthase is the nuclear membrane-embedded enzyme responsible for LTC(4) biosynthesis, catalyzing the conjugation of two substrates that have considerably different water solubility; that amphipathic LTA(4) as a derivative of arachidonic acid and a water-soluble glutathione (GSH). A previous crystal structure revealed important details of GSH binding and implied a GSH activating function for Arg-104. In addition, Arg-31 was also proposed to participate in the catalysis based on the putative LTA(4) binding model. In this study enzymatic assay with mutant enzymes demonstrates that Arg-104 is required for the binding and activation of GSH and that Arg-31 is needed for catalysis probably by activating the epoxide group of LTA(4).

PubMed: 21454538
DOI: 10.1074/jbc.M110.150177

実験手法

X-RAY DIFFRACTION (1.9 Å)