3P9U

Crystal structure of TetX2 from Bacteroides thetaiotaomicron with substrate analogue

Summary for 3P9U

• Entry DOI: 10.2210/pdb3p9u/pdb
• Descriptor: TetX2 protein, FLAVIN-ADENINE DINUCLEOTIDE, SULFATE ION, ... (4 entities in total)
• Functional Keywords: oxidoreductase, monooxygenase, tetracycline degradation
• Biological source: Bacteroides thetaiotaomicron
• Total number of polymer chains: 4
• Total formula weight: 175718.47

Authors

Walkiewicz, K.,Davlieva, M.,Sun, C.,Lau, K.,Shamoo, Y. (deposition date: 2010-10-18, release date: 2011-04-27, Last modification date: 2024-11-20)

Primary citation

Walkiewicz, K.,Davlieva, M.,Wu, G.,Shamoo, Y.
Crystal structure of Bacteroides thetaiotaomicron TetX2: a tetracycline degrading monooxygenase at 2.8 A resolution.
Proteins, 79:2335-2340, 2011

PubMed Abstract: We have determined the structure of TetX2 at 2.8 Å resolution, and shown that it is a class A flavin dependent oxidoreductase. TetX2 has broad activity against a range of tetracyclines including one of the most recent tetracyclines, tigecycline (Tygacil). Comparison of TetX2 with that of the weakly homologous para-hydroxybenzoate hydroxylase (PHBH) (21% identity) shows substantial differences among residues at the substrate binding site although FAD is positioned in a similar conformation between the two enzymes and is poised to carry out catalysis.

PubMed: 21590745
DOI: 10.1002/prot.23052

Experimental method

X-RAY DIFFRACTION (2.81 Å)