3P74

H181N mutant of pentaerythritol tetranitrate reductase containing a C-terminal His8-tag

Summary for 3P74

• Entry DOI: 10.2210/pdb3p74/pdb
• Related: 1H50
• Descriptor: Pentaerythritol tetranitrate reductase, FLAVIN MONONUCLEOTIDE (3 entities in total)
• Functional Keywords: old yellow enzyme family, alpha/beta barrel, oxidoreductase
• Biological source: Enterobacter cloacae
• Total number of polymer chains: 1
• Total formula weight: 41072.66

Authors

Toogood, H.S.,Scrutton, N.S. (deposition date: 2010-10-12, release date: 2011-03-23, Last modification date: 2024-02-21)

• Primary citation: Toogood, H.S.,Fryszkowska, A.,Hulley, M.,Sakuma, M.,Mansell, D.,Stephens, G.M.,Gardiner, J.M.,Scrutton, N.S.; A Site-Saturated Mutagenesis Study of Pentaerythritol Tetranitrate Reductase Reveals that Residues 181 and 184 Influence Ligand Binding, Stereochemistry and Reactivity.; Chembiochem, 12:738-749, 2011; PubMed: 21374779; DOI: 10.1002/cbic.201000662

Experimental method

X-RAY DIFFRACTION (1.2 Å)