3P6B

The crystal structure of CelK CBM4 from Clostridium thermocellum

3P6B の概要

• エントリーDOI: 10.2210/pdb3p6b/pdb
• 関連するPDBエントリー: 3K4Z
• 分子名称: Cellulose 1,4-beta-cellobiosidase, CALCIUM ION, ACETATE ION, ... (6 entities in total)
• 機能のキーワード: beta-sandwich, carbohydrate binding, sugar binding protein
• 由来する生物種: Clostridium thermocellum
• 細胞内の位置: Secreted: P0C2S1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48460.65

構造登録者

Alahuhta, P.M.,Luo, Y.,Lunin, V.V. (登録日: 2010-10-11, 公開日: 2011-08-24, 最終更新日: 2023-09-06)

主引用文献

Alahuhta, M.,Luo, Y.,Ding, S.Y.,Himmel, M.E.,Lunin, V.V.
Structure of CBM4 from Clostridium thermocellum cellulase K.
Acta Crystallogr.,Sect.F, 67:527-530, 2011

PubMed Abstract: Here, a 2.0 Å resolution X-ray structure of Clostridium thermocellum cellulase K family 4 carbohydrate-binding module (CelK CBM4) is reported. The resulting structure was refined to an R factor of 0.212 and an R(free) of 0.274. Structural analysis shows that this new structure is very similar to the previously solved structure of C. thermocellum CbhA CBM4. Most importantly, these data support the previously proposed notion of an extended binding pocket using a novel tryptophan-containing loop that may be highly conserved in clostridial CBM4 proteins.

PubMed: 21543854
DOI: 10.1107/S1744309111003307

実験手法

X-RAY DIFFRACTION (2 Å)