3P30

crystal structure of the cluster II Fab 1281 in complex with HIV-1 gp41 ectodomain

3P30 の概要

• エントリーDOI: 10.2210/pdb3p30/pdb
• 分子名称: HIV-1 gp41, 1281 Fab light chain, 1281 Fab heavy chain (3 entities in total)
• 機能のキーワード: hiv, gp41, cluster 2, fab, 6-helix bundle, immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 57777.54

構造登録者

Frey, G.,Chen, J.,Rits-Volloch, S.,Freeman, M.M.,Zolla-Pazner, S.,Chen, B. (登録日: 2010-10-04, 公開日: 2010-11-17, 最終更新日: 2024-11-20)

主引用文献

Frey, G.,Chen, J.,Rits-Volloch, S.,Freeman, M.M.,Zolla-Pazner, S.,Chen, B.
Distinct conformational states of HIV-1 gp41 are recognized by neutralizing and non-neutralizing antibodies.
Nat.Struct.Mol.Biol., 17:1486-1491, 2010

PubMed Abstract: HIV-1 envelope glycoprotein gp41 undergoes large conformational changes to drive fusion of viral and target cell membranes, adopting at least three distinct conformations during the viral entry process. Neutralizing antibodies against gp41 block HIV-1 infection by targeting gp41's membrane-proximal external region in a fusion-intermediate state. Here we report biochemical and structural evidence that non-neutralizing antibodies, capable of binding with high affinity to an immunodominant segment adjacent to the neutralizing epitopes in the membrane-proximal region, recognize a gp41 conformation that exists only when membrane fusion is complete. We propose that these non-neutralizing antibodies are induced in HIV-1-infected individuals by gp41 in a triggered, postfusion form and contribute to production of ineffective humoral responses. These results have important implications for gp41-based vaccine design.

PubMed: 21076402
DOI: 10.1038/nsmb.1950

実験手法

X-RAY DIFFRACTION (3.3 Å)