3OZP

Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 complexed with PUGNAc

Summary for 3OZP

• Entry DOI: 10.2210/pdb3ozp/pdb
• Related: 3OZO
• Descriptor: N-acetylglucosaminidase, O-(2-ACETAMIDO-2-DEOXY D-GLUCOPYRANOSYLIDENE) AMINO-N-PHENYLCARBAMATE (3 entities in total)
• Functional Keywords: beta-n-acetyl-d-hexosaminidase, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• Biological source: Ostrinia furnacalis (Asian corn borer)
• Total number of polymer chains: 1
• Total formula weight: 65905.23

Authors

Zhang, H.,Liu, T.,Liu, F.,Shen, X.,Yang, Q. (deposition date: 2010-09-27, release date: 2011-08-17, Last modification date: 2024-10-30)

Primary citation

Liu, T.,Zhang, H.,Liu, F.,Chen, L.,Shen, X.,Yang, Q.
Active-pocket size differentiating insectile from bacterial chitinolytic beta-N-acetyl-D-hexosaminidases
Biochem.J., 438:467-474, 2011

PubMed Abstract: Chitinolytic β-N-acetyl-D-hexosaminidase is a branch of the GH20 (glycoside hydrolase family 20) β-N-acetyl-D-hexosaminidases that is only distributed in insects and micro-organisms, and is therefore a potential target for the action of insecticides. PUGNAc [O-(2-acetamido-2-deoxy-D-glucopyransylidene)-amino-N-phenylcarbamate] was initially identified as an inhibitor against GH20 β-N-acetyl-D-hexosaminidases. So far no crystal structure of PUGNAc in complex with any GH20 β-N-acetyl-D-hexosaminidase has been reported. We show in the present study that the sensitivities of chitinolytic β-N-acetyl-D-hexosaminidases towards PUGNAc can vary by 100-fold, with the order being OfHex1 (Ostrinia furnacalis β-N-acetyl-D-hexosaminidase)PubMed: 21692744
DOI: 10.1042/BJ20110390

Experimental method

X-RAY DIFFRACTION (2 Å)