3OVL

Structure of an amyloid forming peptide VQIVYK from the TAU protein in complex with orange G

3OVL の概要

• エントリーDOI: 10.2210/pdb3ovl/pdb
• 関連するPDBエントリー: 2ON9 3FQP
• 分子名称: Microtubule-associated protein, ZINC ION, ACETIC ACID, ... (5 entities in total)
• 機能のキーワード: amyloid-like protofibril in complex with a small-molecule binder, protein fibril
• 細胞内の位置: Cytoplasm, cytosol: P10636
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1349.19

構造登録者

Landau, M.,Eisenberg, D. (登録日: 2010-09-16, 公開日: 2011-07-06, 最終更新日: 2024-02-21)

主引用文献

Landau, M.,Sawaya, M.R.,Faull, K.F.,Laganowsky, A.,Jiang, L.,Sievers, S.A.,Liu, J.,Barrio, J.R.,Eisenberg, D.
Towards a pharmacophore for amyloid.
Plos Biol., 9:e1001080-e1001080, 2011

PubMed Abstract: Diagnosing and treating Alzheimer's and other diseases associated with amyloid fibers remains a great challenge despite intensive research. To aid in this effort, we present atomic structures of fiber-forming segments of proteins involved in Alzheimer's disease in complex with small molecule binders, determined by X-ray microcrystallography. The fiber-like complexes consist of pairs of β-sheets, with small molecules binding between the sheets, roughly parallel to the fiber axis. The structures suggest that apolar molecules drift along the fiber, consistent with the observation of nonspecific binding to a variety of amyloid proteins. In contrast, negatively charged orange-G binds specifically to lysine side chains of adjacent sheets. These structures provide molecular frameworks for the design of diagnostics and drugs for protein aggregation diseases.

PubMed: 21695112
DOI: 10.1371/journal.pbio.1001080

実験手法

X-RAY DIFFRACTION (1.81 Å)