3OUX

Structure of beta-catenin with phosphorylated Lef-1

3OUX の概要

• エントリーDOI: 10.2210/pdb3oux/pdb
• 関連するPDBエントリー: 3OUW
• 分子名称: Catenin beta-1, Lymphoid enhancer-binding factor 1 (3 entities in total)
• 機能のキーワード: wnt/beta-catenin signaling pathway, tcf, lef, transcription factors, phosphorylation, protein binding
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Cytoplasm: Q02248; Nucleus: P27782
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66008.95

構造登録者

Weis, W.I.,Sun, J. (登録日: 2010-09-15, 公開日: 2010-11-24, 最終更新日: 2024-10-30)

主引用文献

Sun, J.,Weis, W.I.
Biochemical and structural characterization of beta-catenin interactions with nonphosphorylated and CK2-phosphorylated Lef-1.
J.Mol.Biol., 405:519-530, 2011

PubMed Abstract: In the Wnt/β-catenin signaling pathway, β-catenin activates target genes through its interactions with the T-cell factor/lymphoid enhancer-binding factor (TCF/Lef) family of transcription factors. The crystal structures of complexes between the β-catenin armadillo domain and the Lef-1 N-terminal domain show that the overall conformation and many of the interactions are similar to other published structures of TCFs bound to β-catenin. However, a second salt bridge in other TCF-β-catenin structures is absent in the structure of β-catenin-Lef-1 complex, indicating that this feature is not obligatory for β-catenin binding. Casein kinase II (CK2) has been shown to act as a positive regulator of Wnt signaling, and Lef-1 is a substrate of CK2. In vitro phosphorylation of purified Lef-1 was used to examine the effect of CK2 on the interaction of Lef-1 with β-catenin. Mass spectrometry data show that CK2 phosphorylation of Lef-1 N-terminal domain results in a single phosphorylation site at Ser40. Isothermal titration calorimetry revealed that β-catenin binds to nonphosphorylated or CK2-phosphorylated Lef-1 with the same affinity, which is consistent with the absence of phospho-Ser40 interactions in the crystal structure of phosphorylated Lef-1 N-terminal domain bound to β-catenin. These data indicate that the effect of CK2 on the Wnt/β-catenin pathway does not appear to be at the level of the Lef-1-β-catenin interaction.

PubMed: 21075118
DOI: 10.1016/j.jmb.2010.11.010

実験手法

X-RAY DIFFRACTION (2.4 Å)