3ONM

Effector binding Domain of LysR-Type transcription factor RovM from Y. pseudotuberculosis

Summary for 3ONM

• Entry DOI: 10.2210/pdb3onm/pdb
• Descriptor: Transcriptional regulator LrhA (2 entities in total)
• Functional Keywords: lysr, rovm, transcription factor, virulence factor, transcription
• Biological source: Yersinia pseudotuberculosis
• Total number of polymer chains: 2
• Total formula weight: 52317.36

Authors

Quade, N.,Diekmann, M.,Haffke, M.,Heroven, A.K.,Dersch, P.,Heinz, D.W. (deposition date: 2010-08-30, release date: 2011-01-26, Last modification date: 2024-02-21)

Primary citation

Quade, N.,Dieckmann, M.,Haffke, M.,Heroven, A.K.,Dersch, P.,Heinz, D.W.
Structure of the effector-binding domain of the LysR-type transcription factor RovM from Yersinia pseudotuberculosis.
Acta Crystallogr.,Sect.D, 67:81-90, 2011

PubMed Abstract: In enteropathogenic Yersinia, the expression of several early-phase virulence factors such as invasin is tightly regulated in response to environmental cues. The responsible regulatory network is complex, involving several regulatory RNAs and proteins such as the LysR-type transcription regulator (LTTR) RovM. In this study, the crystal structure of the effector-binding domain (EBD) of RovM, the first LTTR protein described as being involved in virulence regulation, was determined at a resolution of 2.4 Å. Size-exclusion chromatography and comparison with structures of full-length LTTRs show that RovM is most likely to adopt a tetrameric arrangement with two distant DNA-binding domains (DBDs), causing the DNA to bend around it. Additionally, a cavity was detected in RovM which could bind small inducer molecules.

PubMed: 21245528
DOI: 10.1107/S0907444910049681

Experimental method

X-RAY DIFFRACTION (2.4 Å)