3OIX
Crystal structure of the putative dihydroorotate dehydrogenase from Streptococcus mutans
3OIX の概要
エントリーDOI | 10.2210/pdb3oix/pdb |
分子名称 | Putative dihydroorotate dehydrogenase; dihydroorotate oxidase, FLAVIN MONONUCLEOTIDE, GLYCEROL, ... (4 entities in total) |
機能のキーワード | tim barrel, oxidoreductase |
由来する生物種 | Streptococcus mutans |
細胞内の位置 | Cytoplasm : Q8DVA1 |
タンパク質・核酸の鎖数 | 4 |
化学式量合計 | 154074.78 |
構造登録者 | |
主引用文献 | Liu, Y.,Gao, Z.-Q.,Liu, C.-P.,Xu, J.-H.,Li, L.-F.,Ji, C.-N.,Su, X.-D.,Dong, Y.-H. Structure of the putative dihydroorotate dehydrogenase from Streptococcus mutans Acta Crystallogr.,Sect.F, 67:182-187, 2011 Cited by PubMed Abstract: Streptococcus mutans is one of the pathogenic species involved in dental caries, especially in the initiation and development stages. Here, the crystal structure of SMU.595, a putative dihydroorotate dehydrogenase (DHOD) from S. mutans, is reported at 2.4 Å resolution. DHOD is a flavin mononucleotide-containing enzyme which catalyzes the oxidation of L-dihydroorotate to orotate, which is the fourth step and the only redox reaction in the de novo biosynthesis of pyrimidine nucleotides. The reductive lysine-methylation procedure was applied in order to improve the diffraction qualities of the crystals. Analysis of the S. mutans DHOD crystal structure shows that this enzyme is a class 1A DHOD and also suggests potential sites that could be exploited for the design of highly specific inhibitors using the structure-based chemotherapeutic design technique. PubMed: 21301083DOI: 10.1107/S1744309110048414 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.399 Å) |
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