3OGM

Structure of COI1-ASK1 in complex with coronatine and the JAZ1 degron

3OGM の概要

• エントリーDOI: 10.2210/pdb3ogm/pdb
• 関連するPDBエントリー: 3OGK 3OGL
• 分子名称: SKP1-like protein 1A, Coronatine-insensitive protein 1, JAZ1 degron peptide, ... (5 entities in total)
• 機能のキーワード: leucine rich repeats, ubiquitin ligase, scf, protein binding
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress); 詳細
• 細胞内の位置: Nucleus: Q39255
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 708814.21

構造登録者

Sheard, L.B.,Tan, X.,Mao, H.,Withers, J.,Ben-Nissan, G.,Hinds, T.R.,Hsu, F.,Sharon, M.,Browse, J.,He, S.Y.,Rizo, J.,Howe, G.A.,Zheng, N. (登録日: 2010-08-17, 公開日: 2010-10-13, 最終更新日: 2024-02-21)

主引用文献

Sheard, L.B.,Tan, X.,Mao, H.,Withers, J.,Ben-Nissan, G.,Hinds, T.R.,Kobayashi, Y.,Hsu, F.F.,Sharon, M.,Browse, J.,He, S.Y.,Rizo, J.,Howe, G.A.,Zheng, N.
Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor.
Nature, 468:400-405, 2010

PubMed Abstract: Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

PubMed: 20927106
DOI: 10.1038/nature09430

実験手法

X-RAY DIFFRACTION (3.34 Å)