3ODQ

Structure of a Crystal Form of Human Methemoglobin Indicative of Fiber Formation

3ODQ の概要

• エントリーDOI: 10.2210/pdb3odq/pdb
• 分子名称: Hemoglobin subunit alpha, Hemoglobin subunit beta, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: structural genomics, psi-2, protein structure initiative, center for high-throughput structural biology, chtsb, oxygen transport, oxygen binding, heme group, red blood cells
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64547.05

構造登録者

Larson, S.B.,Day, J.S.,Nguyen, C.,Cudney, R.,Mcpherson, A.,Center for High-Throughput Structural Biology (CHTSB) (登録日: 2010-08-11, 公開日: 2010-12-01, 最終更新日: 2023-09-06)

主引用文献

Larson, S.B.,Day, J.S.,Nguyen, C.,Cudney, R.,McPherson, A.
Structure of a crystal form of human methemoglobin indicative of fiber formation.
Acta Crystallogr.,Sect.D, 66:1316-1322, 2010

PubMed Abstract: Human methemoglobin was crystallized in a unique unit cell and its structure was solved by molecular replacement. The hexagonal unit cell has unit-cell parameters a = b = 54.6, c = 677.4 Å, with symmetry consistent with space group P6₁22. The unit cell has the second highest aspect ratio of all unit cells contained in the PDB. The 12 molecules in the unit cell describe a right-handed helical filament having no polarity, which is different from the filament composed of HbS fibers, which is the only other well characterized fiber of human hemoglobin. The filaments reported here can be related to canonical sickle-cell hemoglobin filaments and to an alternative sickle-cell filament deduced from fiber diffraction by slight modifications of intermolecular contacts.

PubMed: 21123872
DOI: 10.1107/S0907444910040370

実験手法

X-RAY DIFFRACTION (3.1 Å)