3O7A

Crystal structure of PHF13 in complex with H3K4me3

3O7A の概要

• エントリーDOI: 10.2210/pdb3o7a/pdb
• 関連するPDBエントリー: 3O70
• 分子名称: PHD finger protein 13 variant, H3K4ME3 HISTONE 11MER-PEPTIDE, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: phf13; zinc finger; phd domain, nuclear protein, structural genomics, structural genomics consortium, sgc, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Chromosome . Nucleus : Q92133
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7574.60

構造登録者

Bian, C.B.,Lam, R.,Xu, C.,Bountra, C.,Arrowsmith, C.H.,Weigelt, J.,Edwards, A.M.,Bochkarev, A.,Min, J.,Structural Genomics Consortium (SGC) (登録日: 2010-07-30, 公開日: 2010-10-06, 最終更新日: 2023-09-06)

主引用文献

Chung, H.R.,Xu, C.,Fuchs, A.,Mund, A.,Lange, M.,Staege, H.,Schubert, T.,Bian, C.,Dunkel, I.,Eberharter, A.,Regnard, C.,Klinker, H.,Meierhofer, D.,Cozzuto, L.,Winterpacht, A.,Di Croce, L.,Min, J.,Will, H.,Kinkley, S.
PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3.
Elife, 5:-, 2016

PubMed Abstract: PHF13 is a chromatin affiliated protein with a functional role in differentiation, cell division, DNA damage response and higher chromatin order. To gain insight into PHF13's ability to modulate these processes, we elucidate the mechanisms targeting PHF13 to chromatin, its genome wide localization and its molecular chromatin context. Size exclusion chromatography, mass spectrometry, X-ray crystallography and ChIP sequencing demonstrate that PHF13 binds chromatin in a multivalent fashion via direct interactions with H3K4me2/3 and DNA, and indirectly via interactions with PRC2 and RNA PolII. Furthermore, PHF13 depletion disrupted the interactions between PRC2, RNA PolII S5P, H3K4me3 and H3K27me3 and resulted in the up and down regulation of genes functionally enriched in transcriptional regulation, DNA binding, cell cycle, differentiation and chromatin organization. Together our findings argue that PHF13 is an H3K4me2/3 molecular reader and transcriptional co-regulator, affording it the ability to impact different chromatin processes.

PubMed: 27223324
DOI: 10.7554/eLife.10607

実験手法

X-RAY DIFFRACTION (1.67 Å)