3O77

The structure of Ca2+ Sensor (Case-16)

3O77 の概要

• エントリーDOI: 10.2210/pdb3o77/pdb
• 関連するPDBエントリー: 3O78 3ek4 3ek7 3ek8 3ekh 3ekj 3evp 3evr 3evu 3evv
• 分子名称: Myosin light chain kinase, smooth muscle,Green fluorescent protein,Green fluorescent protein,Calmodulin-1, CALCIUM ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: circular permutated green fluorescent protein, genetically encoded, fluorescent calcium indicator protein, calcium sensor, gfp calmodulin m13-peptide, m13-peptide, gyg naturally modified tripeptide acts as chromophore, fluorescent protein
• 由来する生物種: Gallus gallus (Chicken); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton, spindle : P0DP23
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46785.59

構造登録者

Leder, L.,Stark, W.,Freuler, F.,Marsh, M.,Meyerhofer, M.,Stettler, T.,Mayr, L.M.,Britanova, O.V.,Strukova, L.A.,Chudakov, D.M. (登録日: 2010-07-30, 公開日: 2010-09-29, 最終更新日: 2024-11-20)

主引用文献

Leder, L.,Stark, W.,Freuler, F.,Marsh, M.,Meyerhofer, M.,Stettler, T.,Mayr, L.M.,Britanova, O.V.,Strukova, L.A.,Chudakov, D.M.,Souslova, E.A.
The structure of Ca2+ sensor Case16 reveals the mechanism of reaction to low Ca2+ concentrations
Sensors (Basel), 10:8143-8160, 2010

PubMed Abstract: Here we report the first crystal structure of a high-contrast genetically encoded circularly permuted green fluorescent protein (cpGFP)-based Ca(2+) sensor, Case16, in the presence of a low Ca(2+) concentration. The structure reveals the positioning of the chromophore within Case16 at the first stage of the Ca(2+)-dependent response when only two out of four Ca(2+)-binding pockets of calmodulin (CaM) are occupied with Ca(2+) ions. In such a "half Ca(2+)-bound state", Case16 is characterized by an incomplete interaction between its CaM-/M13-domains. We also report the crystal structure of the related Ca(2+) sensor Case12 at saturating Ca(2+) concentration. Based on this structure, we postulate that cpGFP-based Ca(2+) sensors can form non-functional homodimers where the CaM-domain of one sensor molecule binds symmetrically to the M13-peptide of the partner sensor molecule. Case12 and Case16 behavior upon addition of high concentrations of free CaM or M13-peptide reveals that the latter effectively blocks the fluorescent response of the sensor. We speculate that the demonstrated intermolecular interaction with endogenous substrates and homodimerization can impede proper functioning of this type of Ca(2+) sensors in living cells.

PubMed: 22163646
DOI: 10.3390/s100908143

実験手法

X-RAY DIFFRACTION (2.35 Å)