3O4M

Crystal structure of porcine pancreatic phospholipase A2 in complex with 1,2-dihydroxybenzene

3O4M の概要

• エントリーDOI: 10.2210/pdb3o4m/pdb
• 関連するPDBエントリー: 2B00 2B03 2B04 3HSW 3L30 3L69
• 分子名称: Phospholipase A2, major isoenzyme, CALCIUM ION, CATECHOL, ... (4 entities in total)
• 機能のキーワード: catechol binding, pla2, pancreatic enzyme, disulfide bond, lipid degradation, lipoprotein, metal-binding, palmitate, pyrrolidone carboxylic acid, pancreas, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Sus scrofa (Pig)
• 細胞内の位置: Secreted: P00592
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14199.98

構造登録者

Dileep, K.V.,Tintu, I.,Karthe, P.,Mandal, P.K.,Haridas, M.,Sadasivan, C. (登録日: 2010-07-27, 公開日: 2010-08-25, 最終更新日: 2024-10-30)

主引用文献

Dileep, K.V.,Tintu, I.,Mandal, P.K.,Karthe, P.,Haridas, M.,Sadasivan, C.
Binding to PLA(2) may contribute to the anti-inflammatory activity of catechol
Chem.Biol.Drug Des., 2011

PubMed Abstract: Inhibiting PLA(2) activity should, in theory, be an effective approach to control the inflammation. Several naturally occurring polyphenolic compounds have been reported as inhibitors of PLA(2) . Among the naturally occurring polyphenols, catechol (1,2-dihydroxybenzene) possesses anti-inflammatory activity. Catechol can inhibit cyclooxygenase and lipo-oxygenase. By means of enzyme kinetic study, it was revealed that catechol can inhibit PLA(2) also. Crystal structure showed that catechol binds to PLA(2) at the opening of the active site cleft. This might stop the entry of substrate into the active site. Hence, catechol can be used as a lead compound for the development of novel anti-inflammatory drugs with PLA(2) as the target.

PubMed: 21995306
DOI: 10.1111/j.1747-0285.2011.01258.x

実験手法

X-RAY DIFFRACTION (2.5 Å)