3O40

Complex of a chimeric alpha/beta-peptide based on the gp41 CHR domain bound to gp41-5

3O40 の概要

• エントリーDOI: 10.2210/pdb3o40/pdb
• 関連するPDBエントリー: 3O3X 3O3Y 3O3Z 3O42 3O43
• 分子名称: gp41-5, chimeric alpha/beta peptide based on gp41 CHR domain sequence, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: hiv fusion inhibitor, mixed alpha-peptide/beta-peptide backbone, viral protein
• 由来する生物種: artificial gene; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27464.07

構造登録者

Horne, W.S.,Johnson, L.M.,Gellman, S.H. (登録日: 2010-07-26, 公開日: 2011-07-27, 最終更新日: 2023-11-15)

主引用文献

Johnson, L.M.,Horne, W.S.,Gellman, S.H.
Broad Distribution of Energetically Important Contacts across an Extended Protein Interface.
J.Am.Chem.Soc., 133:10038-10041, 2011

PubMed Abstract: Infection of cells by HIV depends upon profound structural rearrangements within the trimeric viral protein gp41. Critical to this process is the formation of a six-helix bundle in which a set of three N-terminal heptad repeat (NHR) helices assemble to form a core displaying long grooves that provide docking sites for three C-terminal heptad repeat (CHR) helices. We report experiments designed to discriminate between two alternative hypotheses regarding the source of affinity between individual CHR helices and the complementary groove: (1) affinity is dominated by interactions of a small cluster of side chains at one end of the CHR helix; or (2) affinity depends upon interactions distributed across the long CHR helix. We have employed two complementary experimental designs, and results from both favor the latter hypothesis.

PubMed: 21644542
DOI: 10.1021/ja203358t

実験手法

X-RAY DIFFRACTION (2.1 Å)