3NR8

Crystal structure of human SHIP2

3NR8 の概要

• エントリーDOI: 10.2210/pdb3nr8/pdb
• 関連するPDBエントリー: 3MTC 3N9V
• 分子名称: Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2, CHLORIDE ION (3 entities in total)
• 機能のキーワード: structural genomics consortium, sgc, phosphatidylinositol-3, 4, 5-trisphosphate 5-phosphatase 2, ship2, inppl1, ship-2, phosphatidylinositol, phosphatase, signalling, magnesium, structural genomics consortium stockholm, magnesium binding, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytosol: O15357
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72523.16

構造登録者

Tresaugues, L.,Welin, M.,Arrowsmith, C.H.,Berglund, H.,Bountra, C.,Collins, R.,Edwards, A.M.,Flodin, S.,Flores, A.,Graslund, S.,Hammarstrom, M.,Johansson, I.,Karlberg, T.,Kol, S.,Kotenyova, T.,Kouznetsova, E.,Moche, M.,Nyman, T.,Persson, C.,Schuler, H.,Schutz, P.,Siponen, M.I.,Thorsell, A.G.,van der Berg, S.,Wahlberg, E.,Weigelt, J.,Nordlund, P.,Structural Genomics Consortium (SGC) (登録日: 2010-06-30, 公開日: 2010-08-25, 最終更新日: 2023-09-06)

主引用文献

Tresaugues, L.,Silvander, C.,Flodin, S.,Welin, M.,Nyman, T.,Graslund, S.,Hammarstrom, M.,Berglund, H.,Nordlund, P.
Structural basis for phosphoinositide substrate recognition, catalysis, and membrane interactions in human inositol polyphosphate 5-phosphatases
Structure, 22:744-755, 2014

PubMed Abstract: SHIP2, OCRL, and INPP5B belong to inositol polyphosphate 5-phophatase subfamilies involved in insulin regulation and Lowes syndrome. The structural basis for membrane recognition, substrate specificity, and regulation of inositol polyphosphate 5-phophatases is still poorly understood. We determined the crystal structures of human SHIP2, OCRL, and INPP5B, the latter in complex with phosphoinositide substrate analogs, which revealed a membrane interaction patch likely to assist in sequestering substrates from the lipid bilayer. Residues recognizing the 1-phosphate of the substrates are highly conserved among human family members, suggesting similar substrate binding modes. However, 3- and 4-phosphate recognition varies and determines individual substrate specificity profiles. The high conservation of the environment of the scissile 5-phosphate suggests a common reaction geometry for all members of the human 5-phosphatase family.

PubMed: 24704254
DOI: 10.1016/j.str.2014.01.013

実験手法

X-RAY DIFFRACTION (2.8 Å)