3NFI

Crystal structure of tandem winged helix domain of RNA polymerase I subunit A49

3NFI の概要

• エントリーDOI: 10.2210/pdb3nfi/pdb
• 関連するPDBエントリー: 3NFF 3NFG 3NFH
• 分子名称: DNA-directed RNA polymerase I subunit RPA49, 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL (3 entities in total)
• 機能のキーワード: winged helix, transcription, rna polymerase, dna binding, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae (yeast)
• 細胞内の位置: Nucleus, nucleolus : Q01080
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 136063.01

構造登録者

Geiger, S.R.,Lorenzen, K.,Schreieck, A.,Hanecker, P.,Kostrewa, D.,Heck, A.J.R.,Cramer, P. (登録日: 2010-06-10, 公開日: 2010-09-08, 最終更新日: 2015-03-25)

主引用文献

Geiger, S.R.,Lorenzen, K.,Schreieck, A.,Hanecker, P.,Kostrewa, D.,Heck, A.J.,Cramer, P.
RNA Polymerase I Contains a TFIIF-Related DNA-Binding Subcomplex.
Mol.Cell, 39:583-594, 2010

PubMed Abstract: The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related to TFIIF and TFIIE. The N-terminal regions of A49 and A34.5 form a dimerization module that stimulates polymerase-intrinsic RNA cleavage and has a fold that resembles the TFIIF core. The C-terminal region of A49 forms a "tandem winged helix" (tWH) domain that binds DNA with a preference for the upstream promoter nontemplate strand and is predicted in TFIIE. Similar domains are predicted in Pol III-specific subunits. Thus, Pol I/III subunits that have no counterparts in Pol II are evolutionarily related to Pol II initiation factors and may have evolved to mediate promoter specificity and transcription processivity.

PubMed: 20797630
DOI: 10.1016/j.molcel.2010.07.028

実験手法

X-RAY DIFFRACTION (1.9 Å)