3NF5

Crystal structure of the C-terminal domain of nuclear pore complex component NUP116 from Candida glabrata

Summary for 3NF5

• Entry DOI: 10.2210/pdb3nf5/pdb
• Descriptor: Nucleoporin NUP116, GLYCEROL (3 entities in total)
• Functional Keywords: nuclear pore complex, nup116, glebs domain, structural genomics, psi-2, protein structure initiative, new york structural genomix research consortium, nysgxrc, new york sgx research center for structural genomics, protein transport
• Biological source: Candida glabrata (yeast)
• Total number of polymer chains: 2
• Total formula weight: 38080.00

Authors

Sampathkumar, P.,Manglicmot, D.,Bain, K.,Gilmore, J.,Gheyi, T.,Rout, M.,Sali, A.,Atwell, S.,Thompson, D.A.,Emtage, J.S.,Wasserman, S.,Sauder, J.M.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (deposition date: 2010-06-09, release date: 2010-08-04, Last modification date: 2024-10-16)

Primary citation

Sampathkumar, P.,Kim, S.J.,Manglicmot, D.,Bain, K.T.,Gilmore, J.,Gheyi, T.,Phillips, J.,Pieper, U.,Fernandez-Martinez, J.,Franke, J.D.,Matsui, T.,Tsuruta, H.,Atwell, S.,Thompson, D.A.,Emtage, J.S.,Wasserman, S.R.,Rout, M.P.,Sali, A.,Sauder, J.M.,Almo, S.C.,Burley, S.K.
Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata.
Proteins, 80:2110-2116, 2012

PubMed Abstract: The nuclear pore complex (NPC), embedded in the nuclear envelope, is a large, dynamic molecular assembly that facilitates exchange of macromolecules between the nucleus and the cytoplasm. The yeast NPC is an eightfold symmetric annular structure composed of ~456 polypeptide chains contributed by ~30 distinct proteins termed nucleoporins. Nup116, identified only in fungi, plays a central role in both protein import and mRNA export through the NPC. Nup116 is a modular protein with N-terminal "FG" repeats containing a Gle2p-binding sequence motif and a NPC targeting domain at its C-terminus. We report the crystal structure of the NPC targeting domain of Candida glabrata Nup116, consisting of residues 882-1034 [CgNup116(882-1034)], at 1.94 Å resolution. The X-ray structure of CgNup116(882-1034) is consistent with the molecular envelope determined in solution by small-angle X-ray scattering. Structural similarities of CgNup116(882-1034) with homologous domains from Saccharomyces cerevisiae Nup116, S. cerevisiae Nup145N, and human Nup98 are discussed.

PubMed: 22544723
DOI: 10.1002/prot.24102

Experimental method

X-RAY DIFFRACTION (1.94 Å)