3NCT

X-ray crystal structure of the bacterial conjugation factor PsiB, a negative regulator of reca

3NCT の概要

• エントリーDOI: 10.2210/pdb3nct/pdb
• 分子名称: Protein psiB (2 entities in total)
• 機能のキーワード: dna binding protein, chaperone
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64508.99

構造登録者

Petrova, V.,Satyshur, K.A.,George, N.P.,McCaslin, D.,Cox, M.M.,Keck, J.L. (登録日: 2010-06-05, 公開日: 2010-07-21, 最終更新日: 2024-11-27)

主引用文献

Petrova, V.,Satyshur, K.A.,George, N.P.,McCaslin, D.,Cox, M.M.,Keck, J.L.
X-ray crystal structure of the bacterial conjugation factor PsiB, a negative regulator of RecA.
J.Biol.Chem., 285:30615-30621, 2010

PubMed Abstract: During bacterial conjugation, genetic material from one cell is transferred to another as single-stranded DNA. The introduction of single-stranded DNA into the recipient cell would ordinarily trigger a potentially deleterious transcriptional response called SOS, which is initiated by RecA protein filaments formed on the DNA. During F plasmid conjugation, however, the SOS response is suppressed by PsiB, an F-plasmid-encoded protein that binds and sequesters free RecA to prevent filament formation. Among the many characterized RecA modulator proteins, PsiB is unique in using sequestration as an inhibitory mechanism. We describe the crystal structure of PsiB from the Escherichia coli F plasmid. The stucture of PsiB is surprisingly similar to CapZ, a eukaryotic actin filament capping protein. Structure-directed neutralization of electronegative surfaces on PsiB abrogates RecA inhibition whereas neutralization of an electropositive surface element enhances PsiB inhibition of RecA. Together, these studies provide a first molecular view of PsiB and highlight its use as a reagent in studies of RecA activity.

PubMed: 20659894
DOI: 10.1074/jbc.M110.152298

実験手法

X-RAY DIFFRACTION (2.2 Å)