3NB3

The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components

3NB3 の概要

• エントリーDOI: 10.2210/pdb3nb3/pdb
• 関連するPDBエントリー: 1qjp 2J1N
• EMDBエントリー: 5201
• 分子名称: Outer membrane protein A, Outer membrane protein C (2 entities in total)
• 機能のキーワード: virus assembly, cementing protein, bacteriophage, sf6, shigella, beta-barrel, outer membrane protein, icosahedral, membrane protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 150154.26

構造登録者

Zhao, H.,Sequeira, R.D.,Galeva, N.A.,Tang, L. (登録日: 2010-06-02, 公開日: 2011-02-16, 最終更新日: 2024-02-21)

主引用文献

Zhao, H.,Sequeira, R.D.,Galeva, N.A.,Tang, L.
The host outer membrane proteins OmpA and OmpC are associated with the Shigella phage Sf6 virion.
Virology, 409:319-327, 2011

PubMed Abstract: Assembly of dsDNA bacteriophage is a precisely programmed process. Potential roles of host cell components in phage assembly haven't been well understood. It was previously reported that two unidentified proteins were present in bacteriophage Sf6 virion (Casjens et al, 2004, J.Mol.Biol. 339, 379-394, Fig. 2A). Using tandem mass spectrometry, we have identified the two proteins as outer membrane proteins (OMPs) OmpA and OmpC from its host Shigella flexneri. The transmission electron cryo-microscopy structure of Sf6 shows significant density at specific sites at the phage capsid inner surface. This density fit well with the characteristic beta-barrel domains of OMPs, thus may be due to the two host proteins. Locations of this density suggest a role in Sf6 morphogenesis reminiscent of phage-encoded cementing proteins. These data indicate a new, OMP-related phage:host linkage, adding to previous knowledge that some lambdoid bacteriophage genomes contain OmpC-like genes that express phage-encoded porins in the lysogenic state.

PubMed: 21071053
DOI: 10.1016/j.virol.2010.10.030

実験手法

ELECTRON MICROSCOPY (19 Å)