3N75

X-ray Crystal Structure of the Escherichia coli Inducible Lysine Decarboxylase LdcI

3N75 の概要

• エントリーDOI: 10.2210/pdb3n75/pdb
• 分子名称: Lysine decarboxylase, inducible, GUANOSINE-5',3'-TETRAPHOSPHATE, HEXAETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: pyridoxal-5'-phosphate dependent decarboxylase, acid stress response, stringent response, guanosine tetraphosphate (ppgpp), lyase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm (Probable): P0A9H3
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 412813.55

構造登録者

Kanjee, U.,Alexopoulos, E.,Pai, E.F.,Houry, W.A. (登録日: 2010-05-26, 公開日: 2011-02-16, 最終更新日: 2023-11-22)

主引用文献

Kanjee, U.,Gutsche, I.,Alexopoulos, E.,Zhao, B.,El Bakkouri, M.,Thibault, G.,Liu, K.,Ramachandran, S.,Snider, J.,Pai, E.F.,Houry, W.A.
Linkage between the bacterial acid stress and stringent responses: the structure of the inducible lysine decarboxylase.
Embo J., 30:931-944, 2011

PubMed Abstract: The Escherichia coli inducible lysine decarboxylase, LdcI/CadA, together with the inner-membrane lysine-cadaverine antiporter, CadB, provide cells with protection against mild acidic conditions (pH∼5). To gain a better understanding of the molecular processes underlying the acid stress response, the X-ray crystal structure of LdcI was determined. The structure revealed that the protein is an oligomer of five dimers that associate to form a decamer. Surprisingly, LdcI was found to co-crystallize with the stringent response effector molecule ppGpp, also known as the alarmone, with 10 ppGpp molecules in the decamer. ppGpp is known to mediate the stringent response, which occurs in response to nutrient deprivation. The alarmone strongly inhibited LdcI enzymatic activity. This inhibition is important for modulating the consumption of lysine in cells during acid stress under nutrient limiting conditions. Hence, our data provide direct evidence for a link between the bacterial acid stress and stringent responses.

PubMed: 21278708
DOI: 10.1038/emboj.2011.5

実験手法

X-RAY DIFFRACTION (2 Å)