3N6M

Crystal structure of EV71 RdRp in complex with GTP

3N6M の概要

• エントリーDOI: 10.2210/pdb3n6m/pdb
• 関連するPDBエントリー: 3N6L 3N6N
• 分子名称: RNA-dependent RNA polymerase, NICKEL (II) ION, GUANOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: ev71, rdrp, gtp, transferase
• 由来する生物種: Human enterovirus 71
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53127.49

構造登録者

Wu, Y.,Lou, Z.Y.,Miao, Y.,Yu, Y.,Rao, Z.H. (登録日: 2010-05-26, 公開日: 2011-06-15, 最終更新日: 2024-03-20)

主引用文献

Wu, Y.,Lou, Z.,Miao, Y.,Yu, Y.,Dong, H.,Peng, W.,Bartlam, M.,Li, X.,Rao, Z.
Structures of EV71 RNA-dependent RNA polymerase in complex with substrate and analogue provide a drug target against the hand-foot-and-mouth disease pandemic in China.
Protein Cell, 1:491-500, 2010

PubMed Abstract: Enterovirus 71 (EV71), one of the major causative agents for hand-foot-and-mouth disease (HFMD), has caused more than 100 deaths among Chinese children since March 2008. The EV71 genome encodes an RNAdependent RNA polymerase (RdRp), denoted 3D(pol), which is central for viral genome replication and is a key target for the discovery of specific antiviral therapeutics. Here we report the crystal structures of EV71 RdRp (3D(pol)) and in complex with substrate guanosine-5'-triphosphate and analog 5-bromouridine-5'-triphosphate best to 2.4 Å resolution. The structure of EV71 RdRp (3D(pol)) has a wider open thumb domain compared with the most closely related crystal structure of poliovirus RdRp. And the EV71 RdRp (3D(pol)) complex with GTP or Br-UTP bounded shows two distinct movements of the polymerase by substrate or analogue binding. The model of the complex with the template:primer derived by superimposition with foot-and-mouth disease virus (FMDV) 3D/RNA complex reveals the likely recognition and binding of template:primer RNA by the polymerase. These results together provide a molecular basis for EV71 RNA replication and reveal a potential target for anti-EV71 drug discovery.

PubMed: 21203964
DOI: 10.1007/s13238-010-0061-7

実験手法

X-RAY DIFFRACTION (2.5 Å)