3N3A

Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli in Complex with Reduced NrdI

3N3A の概要

• エントリーDOI: 10.2210/pdb3n3a/pdb
• 関連するPDBエントリー: 3N37 3N38 3N39 3N3B
• 分子名称: Ribonucleoside-diphosphate reductase 2 subunit beta, Protein nrdI, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: ribonucleotide reductase, four-helix bundle, dimanganese cluster, flavoprotein, oxidoreductase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 108515.39

構造登録者

Boal, A.K.,Cotruvo Jr., J.A.,Stubbe, J.,Rosenzweig, A.C. (登録日: 2010-05-19, 公開日: 2010-08-18, 最終更新日: 2023-09-06)

主引用文献

Boal, A.K.,Cotruvo, J.A.,Stubbe, J.,Rosenzweig, A.C.
Structural basis for activation of class Ib ribonucleotide reductase.
Science, 329:1526-1530, 2010

PubMed Abstract: The class Ib ribonucleotide reductase of Escherichia coli can initiate reduction of nucleotides to deoxynucleotides with either a Mn(III)2-tyrosyl radical (Y•) or a Fe(III)2-Y• cofactor in the NrdF subunit. Whereas Fe(III)2-Y• can self-assemble from Fe(II)2-NrdF and O2, activation of Mn(II)2-NrdF requires a reduced flavoprotein, NrdI, proposed to form the oxidant for cofactor assembly by reduction of O2. The crystal structures reported here of E. coli Mn(II)2-NrdF and Fe(II)2-NrdF reveal different coordination environments, suggesting distinct initial binding sites for the oxidants during cofactor activation. In the structures of Mn(II)2-NrdF in complex with reduced and oxidized NrdI, a continuous channel connects the NrdI flavin cofactor to the NrdF Mn(II)2 active site. Crystallographic detection of a putative peroxide in this channel supports the proposed mechanism of Mn(III)2-Y• cofactor assembly.

PubMed: 20688982
DOI: 10.1126/science.1190187

実験手法

X-RAY DIFFRACTION (1.99 Å)