3N0B

TM0449 mutant crystals grown in loops/micromounts

3N0B の概要

• エントリーDOI: 10.2210/pdb3n0b/pdb
• 関連するPDBエントリー: 3MZQ 3MZR 3N02 3N03 3N0C
• 分子名称: Thymidylate synthase thyX, 2'-DEOXYURIDINE 5'-MONOPHOSPHATE, FLAVIN-ADENINE DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: crystals in loops, thyx, fdts, transferase
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 113624.74

構造登録者

Mathews, I.I. (登録日: 2010-05-13, 公開日: 2011-05-25, 最終更新日: 2023-09-06)

主引用文献

Berger, M.A.,Decker, J.H.,Mathews, I.I.
Diffraction study of protein crystals grown in cryoloops and micromounts.
J.Appl.Crystallogr., 43:1513-1518, 2010

PubMed Abstract: Protein crystals are usually grown in hanging or sitting drops and generally get transferred to a loop or micromount for cryocooling and data collection. This paper describes a method for growing crystals on cryoloops for easier manipulation of the crystals for data collection. This study also investigates the steps for the automation of this process and describes the design of a new tray for the method. The diffraction patterns and the structures of three proteins grown by both the new method and the conventional hanging-drop method are compared. The new setup is optimized for the automation of the crystal mounting process. Researchers could prepare nanolitre drops under ordinary laboratory conditions by growing the crystals directly in loops or micromounts. As has been pointed out before, higher levels of supersaturation can be obtained in very small volumes, and the new method may help in the exploration of additional crystallization conditions.

PubMed: 22477781
DOI: 10.1107/S0021889810040409

実験手法

X-RAY DIFFRACTION (2.3 Å)