3MZI

Crystallographic structure of the pseudo-Signaling State of the BLUF Photoreceptor PixD (slr1694) Y8F mutant

3MZI の概要

• エントリーDOI: 10.2210/pdb3mzi/pdb
• 関連するPDBエントリー: 2HFN 2HFO
• 分子名称: Activator of photopigment and puc expression, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: bluf (blue-light using fad) domain, signaling state, phototaxis, protein binding
• 由来する生物種: Synechocystis sp. PCC 6803
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 106788.49

構造登録者

Yuan, H.,Bauer, C.E. (登録日: 2010-05-12, 公開日: 2011-08-31, 最終更新日: 2023-09-06)

主引用文献

Yuan, H.,Dragnea, V.,Wu, Q.,Gardner, K.H.,Bauer, C.E.
Mutational and Structural Studies of the PixD BLUF Output Signal That Affects Light-Regulated Interactions with PixE.
Biochemistry, 50:6365-6375, 2011

PubMed Abstract: PixD (Slr1694) is a BLUF (blue-light-using FAD) photoreceptor used by the cyanobacterium Synechocystis sp. PCC6803 to control phototaxis toward blue light. In this study, we probe the involvement of a conserved Tyr8-Gln50-Met93 triad in promoting an output signal upon blue light excitation of the bound flavin. Analysis of acrylamide quenching of Trp91 fluorescence shows that the side chain of this residue remains partially solvent exposed in both the lit and dark states. Mutational analysis demonstrates that substitution mutations at Tyr8 and Gln50 result in the loss of the photocycle while a mutation of Met93 does not appreciably disturb the formation of the light-excited state and only minimally accelerates its decay from 5.7 to 4.5 s. However, mutations of Tyr8, Gln50, and Met93 disrupt the ability of PixD dimers to interact with PixE to form a higher-order PixD(10)-PixE(5) complex, which is indicative of a lit conformational state. Solution nuclear magnetic resonance spectroscopy and X-ray crystallographic analyses confirm that a Tyr8 to Phe mutation is locked in a pseudo-light-excited state revealing flexible areas in PixD that likely constitute part of an output signal upon light excitation of wild-type PixD.

PubMed: 21688827
DOI: 10.1021/bi200701d

実験手法

X-RAY DIFFRACTION (2.304 Å)