3MSV

The hypoxic regulator of sterol synthesis Nro1 is a nuclear import adaptor

Summary for 3MSV

• Entry DOI: 10.2210/pdb3msv/pdb
• Descriptor: Nuclear import adaptor, Nro1 (2 entities in total)
• Functional Keywords: helix repeats, heat repeats, protein binding
• Biological source: Schizosaccharomyces pombe (Fission yeast)
• Cellular location: Cytoplasm: Q9USJ7
• Total number of polymer chains: 2
• Total formula weight: 88972.68

Authors

Yeh, T.L.,Amzel, L.M.,Bianchet, M.A. (deposition date: 2010-04-29, release date: 2011-04-20, Last modification date: 2024-02-21)

Primary citation

Yeh, T.L.,Lee, C.Y.,Amzel, L.M.,Espenshade, P.J.,Bianchet, M.A.
The hypoxic regulator of sterol synthesis nro1 is a nuclear import adaptor.
Structure, 19:503-514, 2011

PubMed Abstract: Fission yeast protein Sre1, the homolog of the mammalian sterol regulatory element-binding protein (SREBP), is a hypoxic transcription factor required for sterol homeostasis and low-oxygen growth. Nro1 regulates the stability of the N-terminal transcription factor domain of Sre1 (Sre1N) by inhibiting the action of the prolyl 4-hydroxylase-like Ofd1 in an oxygen-dependent manner. The crystal structure of Nro1 determined at 2.2 Å resolution shows an all-α-helical fold that can be divided into two domains: a small N-terminal domain, and a larger C-terminal HEAT-repeat domain. Follow-up studies showed that Nro1 defines a new class of nuclear import adaptor that functions both in Ofd1 nuclear localization and in the oxygen-dependent inhibition of Ofd1 to control the hypoxic response.

PubMed: 21481773
DOI: 10.1016/j.str.2011.01.017

Experimental method

X-RAY DIFFRACTION (2.18 Å)