3MQI

Human early B-cell factor 1 (EBF1) IPT/TIG domain

3MQI の概要

• エントリーDOI: 10.2210/pdb3mqi/pdb
• 分子名称: Transcription factor COE1, ETHYL MERCURY ION, trimethylamine oxide, ... (4 entities in total)
• 機能のキーワード: immunoglobulin like fold, structural genomics, structural genomics consortium, sgc, transcription activator
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Potential): Q9UH73
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 32905.24

構造登録者

Siponen, M.I.,Lehtio, L.,Arrowsmith, C.H.,Bountra, C.,Collins, R.,Edwards, A.M.,Flodin, S.,Flores, A.,Graslund, S.,Hammarstrom, M.,Johansson, I.,Karlberg, T.,Kotenyova, T.,Moche, M.,Nordlund, P.,Nyman, T.,Persson, C.,Schueler, H.,Schutz, P.,Svensson, L.,Thorsell, A.G.,Tresaugues, L.,Van Den Berg, S.,Wahlberg, E.,Weigelt, J.,Welin, M.,Wisniewska, M.,Berglund, H.,Structural Genomics Consortium (SGC) (登録日: 2010-04-28, 公開日: 2010-05-26, 最終更新日: 2024-02-21)

主引用文献

Siponen, M.I.,Wisniewska, M.,Lehtio, L.,Johansson, I.,Svensson, L.,Raszewski, G.,Nilsson, L.,Sigvardsson, M.,Berglund, H.
Structural Determination of Functional Domains in Early B-cell Factor (EBF) Family of Transcription Factors Reveals Similarities to Rel DNA-binding Proteins and a Novel Dimerization Motif.
J.Biol.Chem., 285:25875-25879, 2010

PubMed Abstract: The early B-cell factor (EBF) transcription factors are central regulators of development in several organs and tissues. This protein family shows low sequence similarity to other protein families, which is why structural information for the functional domains of these proteins is crucial to understand their biochemical features. We have used a modular approach to determine the crystal structures of the structured domains in the EBF family. The DNA binding domain reveals a striking resemblance to the DNA binding domains of the Rel homology superfamily of transcription factors but contains a unique zinc binding structure, termed zinc knuckle. Further the EBF proteins contain an IPT/TIG domain and an atypical helix-loop-helix domain with a novel type of dimerization motif. The data presented here provide insights into unique structural features of the EBF proteins and open possibilities for detailed molecular investigations of this important transcription factor family.

PubMed: 20592035
DOI: 10.1074/jbc.C110.150482

実験手法

X-RAY DIFFRACTION (2.3 Å)