3MQ9

Crystal Structure of Ectodomain Mutant of BST-2/Tetherin/CD317 Fused to MBP

3MQ9 の概要

• エントリーDOI: 10.2210/pdb3mq9/pdb
• 関連するPDBエントリー: 3MQ7 3MQB 3MQC
• 分子名称: Bone marrow stromal antigen 2 fused to Maltose-binding periplasmic protein (2 entities in total)
• 機能のキーワード: hiv, antiviral protein
• 由来する生物種: Escherichia coli (bacteria, human); 詳細
• 細胞内の位置: Golgi apparatus, trans-Golgi network: Q10589
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 417547.41

構造登録者

Xiong, Y.,Yang, H.,Wang, J.,Meng, W. (登録日: 2010-04-27, 公開日: 2010-10-27, 最終更新日: 2024-10-30)

主引用文献

Yang, H.,Wang, J.,Jia, X.,McNatt, M.W.,Zang, T.,Pan, B.,Meng, W.,Wang, H.W.,Bieniasz, P.D.,Xiong, Y.
Structural insight into the mechanisms of enveloped virus tethering by tetherin.
Proc.Natl.Acad.Sci.USA, 107:18428-18432, 2010

PubMed Abstract: Tetherin/BST2 is a type-II membrane protein that inhibits the release of a range of enveloped viruses, including HIV-1. Here we report three crystal structures of human tetherin, including the full-length ectodomain, a triple cysteine mutant and an ectodomain truncation. These structures show that tetherin forms a continuous alpha helix encompassing almost the entire ectodomain. Tetherin helices dimerize into parallel coiled coils via interactions throughout the C-terminal portion of the ectodomain. A comparison of the multiple structures of the tetherin dimer reveals inherent constrained flexibility at two hinges positioned at residues A88 and G109. In the crystals, two tetherin ectodomain dimers associate into a tetramer by forming an antiparallel four-helix bundle at their N termini. However, mutagenesis studies suggest that the tetrametric form of tetherin, although potentially contributing to, is not essential for its antiviral activity. Nonetheless, the structural and chemical properties of the N terminus of the ectodomain are important for optimal tethering function. This study provides detailed insight into the mechanisms by which this broad-spectrum antiviral restriction factor can function.

PubMed: 20940320
DOI: 10.1073/pnas.1011485107

実験手法

X-RAY DIFFRACTION (2.8 Å)