3MQ1

Crystal Structure of Dust Mite Allergen Der p 5

Summary for 3MQ1

• Entry DOI: 10.2210/pdb3mq1/pdb
• Descriptor: Mite allergen Der p 5, (4S)-2-METHYL-2,4-PENTANEDIOL, (4R)-2-METHYLPENTANE-2,4-DIOL, ... (5 entities in total)
• Functional Keywords: allergen, dust mite
• Biological source: Dermatophagoides pteronyssinus (European house dust mite)
• Total number of polymer chains: 6
• Total formula weight: 76971.55

Authors

Mueller, G.A.,Gosavi, R.A.,Krahn, J.M.,Edwards, L.L.,Cuneo, M.J.,Glesner, J.,Pomes, A.,Chapman, M.D.,London, R.E.,Pedersen, L.C. (deposition date: 2010-04-27, release date: 2010-06-02, Last modification date: 2024-02-21)

Primary citation

Mueller, G.A.,Gosavi, R.A.,Krahn, J.M.,Edwards, L.L.,Cuneo, M.J.,Glesner, J.,Pomes, A.,Chapman, M.D.,London, R.E.,Pedersen, L.C.
Der p 5 crystal structure provides insight into the group 5 dust mite allergens.
J.Biol.Chem., 285:25394-25401, 2010

PubMed Abstract: Group 5 allergens from house dust mites elicit strong IgE antibody binding in mite-allergic patients. The structure of Der p 5 was determined by x-ray crystallography to better understand the IgE epitopes, to investigate the biologic function in mites, and to compare with the conflicting published Blo t 5 structures, designated 2JMH and 2JRK in the Protein Data Bank. Der p 5 is a three-helical bundle similar to Blo t 5, but the interactions of the helices are more similar to 2JMH than 2JRK. The crystallographic asymmetric unit contains three dimers of Der p 5 that are not exactly alike. Solution scattering techniques were used to assess the multimeric state of Der p 5 in vitro and showed that the predominant state was monomeric, similar to Blo t 5, but larger multimeric species are also present. In the crystal, the formation of the Der p 5 dimer creates a large hydrophobic cavity of approximately 3000 A(3) that could be a ligand-binding site. Many allergens are known to bind hydrophobic ligands, which are thought to stimulate the innate immune system and have adjuvant-like effects on IgE-mediated inflammatory responses.

PubMed: 20534590
DOI: 10.1074/jbc.M110.128306

Experimental method

X-RAY DIFFRACTION (2.8 Å)