3MPP

Botulinum Neurotoxin Type G Receptor Binding Domain

Summary for 3MPP

• Entry DOI: 10.2210/pdb3mpp/pdb
• Descriptor: Botulinum neurotoxin type G (2 entities in total)
• Functional Keywords: beta barrel beta trefoil, toxin
• Biological source: Clostridium botulinum
• Cellular location: Secreted (By similarity): Q60393
• Total number of polymer chains: 1
• Total formula weight: 50678.64

Authors

Schmitt, J.M.,Lacy, D.B. (deposition date: 2010-04-27, release date: 2010-06-16, Last modification date: 2023-09-06)

Primary citation

Schmitt, J.,Karalewitz, A.,Benefield, D.A.,Mushrush, D.J.,Pruitt, R.N.,Spiller, B.W.,Barbieri, J.T.,Lacy, D.B.
Structural analysis of botulinum neurotoxin type G receptor binding .
Biochemistry, 49:5200-5205, 2010

PubMed Abstract: Botulinum neurotoxin (BoNT) binds peripheral neurons at the neuromuscular junction through a dual-receptor mechanism that includes interactions with ganglioside and protein receptors. The receptor identities vary depending on BoNT serotype (A-G). BoNT/B and BoNT/G bind the luminal domains of synaptotagmin I and II, homologous synaptic vesicle proteins. We observe conditions under which BoNT/B binds both Syt isoforms, but BoNT/G binds only SytI. Both serotypes bind ganglioside G(T1b). The BoNT/G receptor-binding domain crystal structure provides a context for examining these binding interactions and a platform for understanding the physiological relevance of different Syt receptor isoforms in vivo.

PubMed: 20507178
DOI: 10.1021/bi100412v

Experimental method

X-RAY DIFFRACTION (1.98 Å)