3MLT

Crystal structure of anti-HIV-1 V3 Fab 2557 in complex with a UG1033 V3 peptide

Summary for 3MLT

• Entry DOI: 10.2210/pdb3mlt/pdb
• Related: 3MLR 3MLS 3MLU 3MLV 3MLW 3MLX 3MLY 3MLZ
• Descriptor: Human monoclonal anti-HIV-1 gp120 V3 antibody 2557 Fab light chain, Human monoclonal anti-HIV-1 gp120 V3 antibody 2557 Fab heavy chain, HIV-1 gp120 third variable region (V3) crown, ... (4 entities in total)
• Functional Keywords: human monoclonal antibody, fab, hiv-1, gp120, third variable loop, antibody-antigen interaction, immune system
• Biological source: Homo sapiens; More
• Total number of polymer chains: 10
• Total formula weight: 195017.24

Authors

Kong, X.-P. (deposition date: 2010-04-18, release date: 2010-07-14, Last modification date: 2017-11-08)

Primary citation

Jiang, X.,Burke, V.,Totrov, M.,Williams, C.,Cardozo, T.,Gorny, M.K.,Zolla-Pazner, S.,Kong, X.P.
Conserved structural elements in the V3 crown of HIV-1 gp120.
Nat.Struct.Mol.Biol., 17:955-961, 2010

PubMed Abstract: Binding of the third variable region (V3) of the HIV-1 envelope glycoprotein gp120 to the cell-surface coreceptors CCR5 or CXCR4 during viral entry suggests that there are conserved structural elements in this sequence-variable region. These conserved elements could serve as epitopes to be targeted by a vaccine against HIV-1. Here we perform a systematic structural analysis of representative human anti-V3 monoclonal antibodies in complex with V3 peptides, revealing that the crown of V3 has four conserved structural elements: an arch, a band, a hydrophobic core and the peptide backbone. These are either unaffected by or are subject to minimal sequence variation. As these regions are targeted by cross-clade neutralizing human antibodies, they provide a blueprint for the design of vaccine immunogens that could elicit broadly cross-reactive protective antibodies.

PubMed: 20622876
DOI: 10.1038/nsmb.1861

Experimental method

X-RAY DIFFRACTION (2.49 Å)