3MJ7

Crystal structure of the complex of JAML and Coxsackie and Adenovirus receptor, CAR

3MJ7 の概要

• エントリーDOI: 10.2210/pdb3mj7/pdb
• 関連するPDBエントリー: 3MJ6 3MJ8 3MJ9
• 分子名称: Junctional adhesion molecule-like, Coxsackievirus and adenovirus receptor homolog, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: immunoglobulin tandem domain, immune receptor complex, cell adhesion, cell junction, glycoprotein, immunoglobulin domain, membrane, transmembrane, costimulation, phosphoprotein, receptor, secreted, tight junction, immune system
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Cell junction (By similarity): Q80UL9; Isoform 1: Cell membrane; Single-pass type I membrane protein (By similarity). Isoform 2: Cell membrane; Single-pass type I membrane protein (By similarity). Isoform 3: Secreted (By similarity): P97792
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57234.05

構造登録者

Verdino, P.,Wilson, I.A. (登録日: 2010-04-12, 公開日: 2010-09-22, 最終更新日: 2024-11-27)

主引用文献

Verdino, P.,Witherden, D.A.,Havran, W.L.,Wilson, I.A.
The molecular interaction of CAR and JAML recruits the central cell signal transducer PI3K.
Science, 329:1210-1214, 2010

PubMed Abstract: Coxsackie and adenovirus receptor (CAR) is the primary cellular receptor for group B coxsackieviruses and most adenovirus serotypes and plays a crucial role in adenoviral gene therapy. Recent discovery of the interaction between junctional adhesion molecule-like protein (JAML) and CAR uncovered important functional roles in immunity, inflammation, and tissue homeostasis. Crystal structures of JAML ectodomain (2.2 angstroms) and its complex with CAR (2.8 angstroms) reveal an unusual immunoglobulin-domain assembly for JAML and a charged interface that confers high specificity. Biochemical and mutagenesis studies illustrate how CAR-mediated clustering of JAML recruits phosphoinositide 3-kinase (P13K) to a JAML intracellular sequence motif as delineated for the alphabeta T cell costimulatory receptor CD28. Thus, CAR and JAML are cell signaling receptors of the immune system with implications for asthma, cancer, and chronic nonhealing wounds.

PubMed: 20813955
DOI: 10.1126/science.1187996

実験手法

X-RAY DIFFRACTION (2.8 Å)