3MFF

1F1E8hu TCR

Summary for 3MFF

• Entry DOI: 10.2210/pdb3mff/pdb
• Descriptor: T cell receptor alpha chain, T cell receptor beta chain, GLYCEROL, ... (6 entities in total)
• Functional Keywords: t-cell receptor, protein metamorphism, alpha constant domain, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 100546.63

Authors

van Boxel, G.I.,Holmes, S.,Fugger, L.,Jones, E.Y. (deposition date: 2010-04-02, release date: 2010-06-02, Last modification date: 2024-10-30)

Primary citation

van Boxel, G.I.,Holmes, S.,Fugger, L.,Jones, E.Y.
An alternative conformation of the T-cell receptor alpha constant region
J.Mol.Biol., 400:828-837, 2010

PubMed Abstract: Alphabeta T-cell receptors (TcRs) play a central role in cellular immune response. They are members of the Ig superfamily, with extracellular regions of the alpha and beta chains each comprising a V-type domain and a C-type domain. We have determined the ectodomain structure of an alphabeta TcR, which recognizes the autoantigen myelin basic protein. The 2.0-A-resolution structure reveals canonical main-chain conformations for the V(alpha), V(beta), and C(beta) domains, but the C(alpha) domain exhibits a main-chain conformation remarkably different from those previously reported for TcR crystal structures. The global IgC-like fold is maintained, but a piston-like rearrangement between BC and DE beta-turns results in beta-strand slippage. This substantial conformational change may represent a signaling intermediate. Our structure is the first example for the Ig fold of the increasingly recognized concept of "metamorphic proteins."

PubMed: 20630474
DOI: 10.1016/j.jmb.2010.05.053

Experimental method

X-RAY DIFFRACTION (2 Å)