3MBX

Crystal structure of chimeric antibody X836

3MBX の概要

• エントリーDOI: 10.2210/pdb3mbx/pdb
• 関連するPDBエントリー: 3L7E
• 分子名称: X836 LIGHT CHAIN, X836 HEAVY CHAIN, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: immunoglobulin fold, monoclonal antibody, immune system
• 由来する生物種: Mus musculus (mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49399.85

構造登録者

Teplyakov, A.,Obmolova, G.,Gilliland, G.L. (登録日: 2010-03-26, 公開日: 2010-06-30, 最終更新日: 2024-11-06)

主引用文献

Teplyakov, A.,Obmolova, G.,Carton, J.M.,Gao, W.,Zhao, Y.,Gilliland, G.L.
On the domain pairing in chimeric antibodies.
Mol.Immunol., 47:2422-2426, 2010

PubMed Abstract: A chimeric antibody was constructed from two unrelated antibodies by combining their heavy and light chains. The "double chimera" consists of the mouse variable regions of different specificity (IL-13 and EMMPRIN) and the constant regions of different origin (mouse and human). The Fab fragment of this chimeric antibody was expressed in mammalian cells, and the crystal structure was determined at 1.6A resolution. Despite a large number of amino acid substitutions in the double chimera with respect to the parent antibodies, the heavy and light chains associate into a stable molecule. Comparison to the structure of one of the parent antibodies reveals that the variable domain interface, as well as the conformation of antigen-binding loops, is preserved without major rearrangements due to conservation of amino acids in key positions. Comparison to the structures of the all-human and all-mouse constant domains indicates a remarkable plasticity of the inter-chain interface that can tolerate residue relocations of up to 6A.

PubMed: 20554002
DOI: 10.1016/j.molimm.2010.05.002

実験手法

X-RAY DIFFRACTION (1.6 Å)