3MAL

Crystal structure of the SDF2-like protein from Arabidopsis thaliana

3MAL の概要

• エントリーDOI: 10.2210/pdb3mal/pdb
• 分子名称: Stromal cell-derived factor 2-like protein, SULFATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: trefoil fold, mir motifs, unfolded protein response, putative sugar binding protein, plant protein
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress, thale-cress)
• 細胞内の位置: Secreted (Probable): Q93ZE8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43983.03

構造登録者

Ravaud, S.,Radzimanowski, J.,Sinning, I. (登録日: 2010-03-24, 公開日: 2010-04-07, 最終更新日: 2023-09-06)

主引用文献

Schott, A.,Ravaud, S.,Keller, S.,Radzimanowski, J.,Viotti, C.,Hillmer, S.,Sinning, I.,Strahl, S.
Arabidopsis stromal-derived Factor2 (SDF2) is a crucial target of the unfolded protein response in the endoplasmic reticulum.
J.Biol.Chem., 285:18113-18121, 2010

PubMed Abstract: Stresses increasing the load of unfolded proteins that enter the endoplasmic reticulum (ER) trigger a protective response termed the unfolded protein response (UPR). Stromal cell-derived factor2 (SDF2)-type proteins are highly conserved throughout the plant and animal kingdoms. In this study we have characterized AtSDF2 as crucial component of the UPR in Arabidopsis thaliana. Using a combination of biochemical and cell biological methods, we demonstrate that SDF2 is induced in response to ER stress conditions causing the accumulation of unfolded proteins. Transgenic reporter plants confirmed induction of SDF2 during ER stress. Under normal growth conditions SDF2 is highly expressed in fast growing, differentiating cells and meristematic tissues. The increased production of SDF2 due to ER stress and in tissues that require enhanced protein biosynthesis and secretion, and its association with the ER membrane qualifies SDF2 as a downstream target of the UPR. Determination of the SDF2 three-dimensional crystal structure at 1.95 A resolution revealed the typical beta-trefoil fold with potential carbohydrate binding sites. Hence, SDF2 might be involved in the quality control of glycoproteins. Arabidopsis sdf2 mutants display strong defects and morphological phenotypes during seedling development specifically under ER stress conditions, thus establishing that SDF2-type proteins play a key role in the UPR.

PubMed: 20378538
DOI: 10.1074/jbc.M110.117176

実験手法

X-RAY DIFFRACTION (1.95 Å)