3M93

Complex crystal structure of Ascaris suum eIF4E-3 with m7G cap

3M93 の概要

• エントリーDOI: 10.2210/pdb3m93/pdb
• 関連するPDBエントリー: 3M94
• 分子名称: Translation initiation factor 4E, Eukaryotic translation initiation factor 4E-binding protein 1, 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: eif4e, berkeley structural genomics center, bsgc, translation
• 由来する生物種: Ascaris suum (Pig roundworm); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24772.13

構造登録者

Liu, W.,Berkeley Structural Genomics Center (BSGC) (登録日: 2010-03-19, 公開日: 2011-07-20, 最終更新日: 2024-03-06)

主引用文献

Liu, W.,Jankowska-Anyszka, M.,Piecyk, K.,Dickson, L.,Wallace, A.,Niedzwiecka, A.,Stepinski, J.,Stolarski, R.,Darzynkiewicz, E.,Kieft, J.,Zhao, R.,Jones, D.N.,Davis, R.E.
Structural basis for nematode eIF4E binding an m2,2,7G-Cap and its implications for translation initiation.
Nucleic Acids Res., 39:8820-8832, 2011

PubMed Abstract: Metazoan spliced leader (SL) trans-splicing generates mRNAs with an m(2,2,7)G-cap and a common downstream SL RNA sequence. The mechanism for eIF4E binding an m²²⁷G-cap is unknown. Here, we describe the first structure of an eIF4E with an m(2,2,7)G-cap and compare it to the cognate m⁷G-eIF4E complex. These structures and Nuclear Magnetic Resonance (NMR) data indicate that the nematode Ascaris suum eIF4E binds the two different caps in a similar manner except for the loss of a single hydrogen bond on binding the m(2,2,7)G-cap. Nematode and mammalian eIF4E both have a low affinity for m(2,2,7)G-cap compared with the m⁷G-cap. Nematode eIF4E binding to the m⁷G-cap, m(2,2,7)G-cap and the m(2,2,7)G-SL 22-nt RNA leads to distinct eIF4E conformational changes. Additional interactions occur between Ascaris eIF4E and the SL on binding the m(2,2,7)G-SL. We propose interactions between Ascaris eIF4E and the SL impact eIF4G and contribute to translation initiation, whereas these interactions do not occur when only the m(2,2,7)G-cap is present. These data have implications for the contribution of 5'-UTRs in mRNA translation and the function of different eIF4E isoforms.

PubMed: 21965542
DOI: 10.1093/nar/gkr650

実験手法

X-RAY DIFFRACTION (2.9 Å)