3LSM

Pyranose 2-oxidase H167A mutant with flavin N(5) sulfite adduct

Summary for 3LSM

• Entry DOI: 10.2210/pdb3lsm/pdb
• Related: 3LSH 3LSI 3LSK
• Descriptor: Pyranose 2-oxidase, (S)-10-((2S,3S,4R)-5-((S)-((S)-(((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHOXY)(HYDROXY)PHOSPHORYLOXY)(HYDROXY)PHOSPHORYLOXY)-2,3,4-TRIHYDROXYPENTYL)-7,8-DIMETHYL-2,4-DIOXO-2,3,4,4A-TETRAHYDROBENZO[G]PTERIDINE-5(10H)-SULFONIC ACID, SULFITE ION, ... (6 entities in total)
• Functional Keywords: oxidoreductase, gmc oxidoreductase, h167a mutant, rossmann fold, phbh fold, homotetramer, covalently linked fad, flavin n(5)-sulfite adduct
• Biological source: Trametes ochracea (White-rot fungus)
• Total number of polymer chains: 2
• Total formula weight: 142022.38

Authors

Tan, T.C.,Spadiut, O.,Divne, C. (deposition date: 2010-02-12, release date: 2010-08-25, Last modification date: 2024-02-21)

• Primary citation: Tan, T.C.,Pitsawong, W.,Wongnate, T.,Spadiut, O.,Haltrich, D.,Chaiyen, P.,Divne, C.; H-bonding and positive charge at the N5/O4 locus are critical for covalent flavin attachment in trametes pyranose 2-oxidase.; J.Mol.Biol., 402:578-594, 2010; PubMed: 20708626; DOI: 10.1016/j.jmb.2010.08.011

Experimental method

X-RAY DIFFRACTION (1.7 Å)