3LRV

The Prp19 WD40 Domain Contains a Conserved Protein Interaction Region Essential for its Function.

3LRV の概要

• エントリーDOI: 10.2210/pdb3lrv/pdb
• 分子名称: Pre-mRNA-splicing factor 19, SULFATE ION (3 entities in total)
• 機能のキーワード: prp19, wd40, e3 ubiquitin ligase, spliceosome, dna damage, dna repair, mrna processing, mrna splicing, nucleus, phosphoprotein, wd repeat, splicing
• 由来する生物種: Saccharomyces cerevisiae (yeast)
• 細胞内の位置: Nucleus: P32523
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39026.69

構造登録者

Vander Kooi, C.W.,Chazin, W.J. (登録日: 2010-02-11, 公開日: 2010-05-26, 最終更新日: 2024-02-21)

主引用文献

Vander Kooi, C.W.,Ren, L.,Xu, P.,Ohi, M.D.,Gould, K.L.,Chazin, W.J.
The Prp19 WD40 domain contains a conserved protein interaction region essential for its function.
Structure, 18:584-593, 2010

PubMed Abstract: Prp19 is a member of the WD40 repeat family of E3 ubiquitin ligases and a conserved eukaryotic RNA splicing factor essential for activation and stabilization of the spliceosome. To understand the role of the WD40 repeat domain of Prp19 we have determined its structure using X-ray crystallography. The domain has a distorted seven bladed WD40 architecture with significant asymmetry due to irregular packing of blades one and seven into the core of the WD40 domain. Structure-based mutagenesis identified a highly conserved surface centered around blade five that is required for the physical interaction between Prp19 and Cwc2, another essential splicing factor. This region is found to be required for Prp19 function and yeast viability. Experiments in vitro and in vivo demonstrate that two molecules of Cwc2 bind to the Prp19 tetramer. These coupled structural and functional studies provide a model for the functional architecture of Prp19.

PubMed: 20462492
DOI: 10.1016/j.str.2010.02.015

実験手法

X-RAY DIFFRACTION (2.6 Å)