3LR7

Ferric horse heart myoglobin, nitrite adduct

3LR7 の概要

• エントリーDOI: 10.2210/pdb3lr7/pdb
• 関連するPDBエントリー: 2frf 3LR9
• 分子名称: Myoglobin, PROTOPORPHYRIN IX CONTAINING FE, NITRITE ION, ... (5 entities in total)
• 機能のキーワード: myoglobin, heme, nitrite, metal-binding, muscle protein, oxygen transport, transport
• 由来する生物種: Equus caballus (domestic horse,equine)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17930.14

構造登録者

Yi, J.,Richter-Addo, G.B. (登録日: 2010-02-10, 公開日: 2010-07-07, 最終更新日: 2023-09-06)

主引用文献

Yi, J.,Orville, A.M.,Skinner, J.M.,Skinner, M.J.,Richter-Addo, G.B.
Synchrotron X-ray-Induced Photoreduction of Ferric Myoglobin Nitrite Crystals Gives the Ferrous Derivative with Retention of the O-Bonded Nitrite Ligand.
Biochemistry, 49:5969-5971, 2010

PubMed Abstract: Exposure of a single crystal of the nitrite adduct of ferric myoglobin (Mb) at 100 K to high-intensity synchrotron X-ray radiation resulted in changes in the UV-vis spectrum that can be attributed to reduction of the ferric compound to the ferrous derivative. We employed correlated single-crystal spectroscopy with crystallography to further characterize this photoproduct. The 1.55 A resolution crystal structure of the photoproduct reveals retention of the O-binding mode for binding of nitrite to the iron center. The data are consistent with cryogenic generation and trapping, at 100 K, of a ferrous d(6) Mb(II)(ONO)* complex by photoreduction of the ferric precursor crystals using high-intensity X-ray radiation.

PubMed: 20568729
DOI: 10.1021/bi100801g

実験手法

X-RAY DIFFRACTION (1.6 Å)