3LQQ

Structure of the CED-4 Apoptosome

3LQQ の概要

• エントリーDOI: 10.2210/pdb3lqq/pdb
• 関連するPDBエントリー: 2a5y 3lqr
• 分子名称: Cell death protein 4, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: ced4, apoptosome, apoptosis, alternative splicing, atp-binding, mitochondrion, nucleotide-binding
• 由来する生物種: Caenorhabditis elegans (nematode)
• 細胞内の位置: Mitochondrion: P30429
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 126969.50

構造登録者

Qi, S.,Pang, Y.,Shi, Y.,Yan, N.,Liu, Q. (登録日: 2010-02-09, 公開日: 2010-04-28, 最終更新日: 2023-11-01)

主引用文献

Qi, S.,Pang, Y.,Hu, Q.,Liu, Q.,Li, H.,Zhou, Y.,He, T.,Liang, Q.,Liu, Y.,Yuan, X.,Luo, G.,Li, H.,Wang, J.,Yan, N.,Shi, Y.
Crystal structure of the Caenorhabditis elegans apoptosome reveals an octameric assembly of CED-4.
Cell(Cambridge,Mass.), 141:446-457, 2010

PubMed Abstract: The CED-4 homo-oligomer or apoptosome is required for initiation of programmed cell death in Caenorhabditis elegans by facilitating autocatalytic activation of the CED-3 caspase zymogen. How the CED-4 apoptosome assembles and activates CED-3 remains enigmatic. Here we report the crystal structure of the complete CED-4 apoptosome and show that it consists of eight CED-4 molecules, organized as a tetramer of an asymmetric dimer via a previously unreported interface among AAA(+) ATPases. These eight CED-4 molecules form a funnel-shaped structure. The mature CED-3 protease is monomeric in solution and forms an active holoenzyme with the CED-4 apoptosome, within which the protease activity of CED-3 is markedly stimulated. Unexpectedly, the octameric CED-4 apoptosome appears to bind only two, not eight, molecules of mature CED-3. The structure of the CED-4 apoptosome reveals shared principles for the NB-ARC family of AAA(+) ATPases and suggests a mechanism for the activation of CED-3.

PubMed: 20434985
DOI: 10.1016/j.cell.2010.03.017

実験手法

X-RAY DIFFRACTION (3.534 Å)