3LI9

Crystal Structure of the extracellular domain of the putative histidine kinase mmHK1S-Z2

3LI9 の概要

• エントリーDOI: 10.2210/pdb3li9/pdb
• 関連するPDBエントリー: 3LI8 3LIA 3LIB 3LIC 3LID 3LIE 3LIF
• 分子名称: Hypothetical sensory transduction histidine kinase, 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total)
• 機能のキーワード: pdc fold, signaling protein
• 由来する生物種: Methanosarcina mazei (Methanosarcina frisia)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33326.83

構造登録者

Zhang, Z.,Hendrickson, W.A. (登録日: 2010-01-24, 公開日: 2010-05-05, 最終更新日: 2024-11-27)

主引用文献

Zhang, Z.,Hendrickson, W.A.
Structural characterization of the predominant family of histidine kinase sensor domains.
J.Mol.Biol., 400:335-353, 2010

PubMed Abstract: Histidine kinase (HK) receptors are used ubiquitously by bacteria to monitor environmental changes, and they are also prevalent in plants, fungi, and other protists. Typical HK receptors have an extracellular sensor portion that detects a signal, usually a chemical ligand, and an intracellular transmitter portion that includes both the kinase domain itself and the site for histidine phosphorylation. While kinase domains are highly conserved, sensor domains are diverse. HK receptors function as dimers, but the molecular mechanism for signal transduction across cell membranes remains obscure. In this study, eight crystal structures were determined from five sensor domains representative of the most populated family, family HK1, found in a bioinformatic analysis of predicted sensor domains from transmembrane HKs. Each structure contains an inserted repeat of PhoQ/DcuS/CitA (PDC) domains, and similarity between sequence and structure is correlated across these and other double-PDC sensor proteins. Three of the five sensors crystallize as dimers that appear to be physiologically relevant, and comparisons between ligated structures and apo-state structures provide insights into signal transmission. Some HK1 family proteins prove to be sensors for chemotaxis proteins or diguanylate cyclase receptors, implying a combinatorial molecular evolution.

PubMed: 20435045
DOI: 10.1016/j.jmb.2010.04.049

実験手法

X-RAY DIFFRACTION (1.7 Å)