3LGB

Crystal Structure of the Fe-S Domain of the yeast DNA primase

3LGB の概要

• エントリーDOI: 10.2210/pdb3lgb/pdb
• 分子名称: DNA primase large subunit, IRON/SULFUR CLUSTER, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (6 entities in total)
• 機能のキーワード: dna primase, fe-s cluster, dna-binding, dna-directed rna polymerase, iron, iron-sulfur, metal-binding, nucleotidyltransferase, primosome, transferase
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47289.06

構造登録者

Sauguet, L.,Pellegrini, L. (登録日: 2010-01-20, 公開日: 2010-04-21, 最終更新日: 2024-11-13)

主引用文献

Sauguet, L.,Klinge, S.,Perera, R.L.,Maman, J.D.,Pellegrini, L.
Shared Active Site Architecture between the Large Subunit of Eukaryotic Primase and DNA Photolyase
PLOS ONE, 5:10083-10083, 2010

PubMed Abstract: DNA synthesis during replication relies on RNA primers synthesised by the primase, a specialised DNA-dependent RNA polymerase that can initiate nucleic acid synthesis de novo. In archaeal and eukaryotic organisms, the primase is a heterodimeric enzyme resulting from the constitutive association of a small (PriS) and large (PriL) subunit. The ability of the primase to initiate synthesis of an RNA primer depends on a conserved Fe-S domain at the C-terminus of PriL (PriL-CTD). However, the critical role of the PriL-CTD in the catalytic mechanism of initiation is not understood.

PubMed: 20404922
DOI: 10.1371/journal.pone.0010083

実験手法

X-RAY DIFFRACTION (1.54 Å)