3LG8

Crystal structure of the C-terminal part of subunit E (E101-206) from Methanocaldococcus jannaschii of A1AO ATP synthase

3LG8 の概要

• エントリーDOI: 10.2210/pdb3lg8/pdb
• 関連するPDBエントリー: 2DM9 2DMA
• 分子名称: A-type ATP synthase subunit E (1 entity in total)
• 機能のキーワード: archaea, peripheral stalk, hydrolase, structural protein, transport protein
• 由来する生物種: Methanocaldococcus jannaschii (Methanococcus jannaschii)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23709.37

構造登録者

Balakrishna, A.M.,Manimekalai, M.S.S.,Hunke, C.,Gayen, S.,Jeyakanthan, J.,Gruber, G. (登録日: 2010-01-19, 公開日: 2010-07-07, 最終更新日: 2023-11-01)

主引用文献

Balakrishna, A.M.,Manimekalai, M.S.S.,Hunke, C.,Gayen, S.,Rossle, M.,Jeyakanthan, J.,Gruber, G.
Crystal and solution structure of the C-terminal part of the Methanocaldococcus jannaschii A1AO ATP synthase subunit E revealed by X-ray diffraction and small-angle X-ray scattering
J.Bioenerg.Biomembr., 42:311-320, 2010

PubMed Abstract: The structure of the C-terminus of subunit E (E(101-206)) of Methanocaldococcus jannaschii A-ATP synthase was determined at 4.1 A. E(101-206) consist of a N-terminal globular domain with three alpha-helices and four antiparallel beta-strands and an alpha-helix at the very C-terminus. Comparison of M. jannaschii E(101-206) with the C-terminus E(81-198) subunit E from Pyrococcus horikoshii OT3 revealed that the kink in the C-terminal alpha-helix of E(81-198), involved in dimer formation, is absent in M. jannaschii E(101-206). Whereas a major dimeric surface interface is present between the P. horikoshii E(81-198) molecules in the asymmetric unit, no such interaction could be found in the M. jannaschii E(101-206) molecules. To verify the oligomeric behaviour, the low resolution structure of the recombinant E(85-206) from M. jannaschii was determined using small angle X-ray scattering. Rigid body modeling of two copies of one of the monomer established a fit with a tail to tail arrangement.

PubMed: 20571891
DOI: 10.1007/s10863-010-9298-3

実験手法

X-RAY DIFFRACTION (4.1 Å)