3LDZ

Crystal structure of human STAM1 VHS domain in complex with ubiquitin

3LDZ の概要

• エントリーDOI: 10.2210/pdb3ldz/pdb
• 分子名称: Ubiquitin, Signal transducing adapter molecule 1 (2 entities in total)
• 機能のキーワード: ubiquitin-binding, cytoplasm, ubl conjugation, endosome, membrane, protein transport, sh3 domain, transport
• 由来する生物種: Bos taurus (bovine); 詳細
• 細胞内の位置: Cytoplasm : Q92783
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 87544.66

構造登録者

Ren, X.,Hurley, J.H. (登録日: 2010-01-13, 公開日: 2010-03-02, 最終更新日: 2024-02-21)

主引用文献

Ren, X.,Hurley, J.H.
VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo.
Embo J., 29:1045-1054, 2010

PubMed Abstract: VHS (Vps27, Hrs, and STAM) domains occur in ESCRT-0 subunits Hrs and STAM, GGA adapters, and other trafficking proteins. The structure of the STAM VHS domain-ubiquitin complex was solved at 2.6 A resolution, revealing that determinants for ubiquitin recognition are conserved in nearly all VHS domains. VHS domains from all classes of VHS-domain containing proteins in yeast and humans, including both subunits of ESCRT-0, bound ubiquitin in vitro. ESCRTs have been implicated in the sorting of Lys63-linked polyubiquitinated cargo. Intact human ESCRT-0 binds Lys63-linked tetraubiquitin 50-fold more tightly than monoubiquitin, though only 2-fold more tightly than Lys48-linked tetraubiquitin. The gain in affinity is attributed to the cooperation of flexibly connected VHS and UIM motifs of ESCRT-0 in avid binding to the polyubiquitin chain. Mutational analysis of all the five ubiquitin-binding sites in yeast ESCRT-0 shows that cooperation between them is required for the sorting of the Lys63-linked polyubiquitinated cargo Cps1 to the vacuole.

PubMed: 20150893
DOI: 10.1038/emboj.2010.6

実験手法

X-RAY DIFFRACTION (2.6 Å)