3LCK

THE KINASE DOMAIN OF HUMAN LYMPHOCYTE KINASE (LCK), ACTIVATED FORM (AUTO-PHOSPHORYLATED ON TYR394)

3LCK の概要

• エントリーDOI: 10.2210/pdb3lck/pdb
• 分子名称: PROTO-ONCOGENE TYROSINE-PROTEIN KINASE, SULFATE ION (3 entities in total)
• 機能のキーワード: tyrosine-protein kinase, atp-binding, phosphorylation, signal transduction
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P06239
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31521.93

構造登録者

Yamaguchi, H.,Hendrickson, W.A. (登録日: 1997-04-08, 公開日: 1997-12-03, 最終更新日: 2024-10-30)

主引用文献

Yamaguchi, H.,Hendrickson, W.A.
Structural basis for activation of human lymphocyte kinase Lck upon tyrosine phosphorylation.
Nature, 384:484-489, 1996

PubMed Abstract: Regulation through phosphorylation is a characteristic of signalling pathways and the lymphocyte kinase Lck (p56lck) both performs phosphorylation and is affected by it. Lck is a Src-family tyrosine kinase expressed in T lymphocytes, where it participates in the cellular immune response. Like all Src homologues, it comprises SH3, SH2 and kinase domains. Lck associates through its distinctive amino-terminal segment with the cytoplasmic tails of either T-cell co-receptor, CD4 or CD8-alpha. Activated Lck phosphorylates T-cell receptor zeta-chains, which then recruit the ZAP70 kinase to promote T-cell activation. Lck is activated by autophosphorylation at Tyr 394 in the activation loop and it is inactive when Tyr 505 near the carboxy terminus is phosphorylated and interacts with its own SH2 domain. Here we report the crystal structure of the Lck tyrosine kinase domain (LCKK) in its activated state at 1.7 A resolution. The structure reveals how a phosphoryl group at Tyr 394 generates a competent active site. Comparisons with other kinase structures indicate that tyrosine phophophorylation and ligand binding may in general elicit two distinct hinge-like movements between the kinase subdomains. From modelling studies, we suggest a basis for inhibition by phosphorylation at Tyr 505.

PubMed: 8945479
DOI: 10.1038/384484a0

実験手法

X-RAY DIFFRACTION (1.7 Å)