3LBN

Ras soaked in Magnesium Acetate

3LBN の概要

• エントリーDOI: 10.2210/pdb3lbn/pdb
• 関連するPDBエントリー: 3K8Y 3K9N 3LBH 3LBI
• 分子名称: GTPase HRas, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: oncoprotein, protein-nucleotide complex, gtp-binding, acetylation, cell membrane, disease mutation, golgi apparatus, lipoprotein, membrane, methylation, nucleotide-binding, palmitate, prenylation, proto-oncogene, s-nitrosylation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Lipid-anchor; Cytoplasmic side: P01112
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19510.38

構造登録者

Holzapfel, G.,Buhrman, G.,Mattos, C. (登録日: 2010-01-08, 公開日: 2010-03-02, 最終更新日: 2023-09-06)

主引用文献

Buhrman, G.,Holzapfel, G.,Fetics, S.,Mattos, C.
Allosteric modulation of Ras positions Q61 for a direct role in catalysis.
Proc.Natl.Acad.Sci.USA, 107:4931-4936, 2010

PubMed Abstract: Ras and its effector Raf are key mediators of the Ras/Raf/MEK/ERK signal transduction pathway. Mutants of residue Q61 impair the GTPase activity of Ras and are found prominently in human cancers. Yet the mechanism through which Q61 contributes to catalysis has been elusive. It is thought to position the catalytic water molecule for nucleophilic attack on the gamma-phosphate of GTP. However, we previously solved the structure of Ras from crystals with symmetry of the space group R32 in which switch II is disordered and found that the catalytic water molecule is present. Here we present a structure of wild-type Ras with calcium acetate from the crystallization mother liquor bound at a site remote from the active site and likely near the membrane. This results in a shift in helix 3/loop 7 and a network of H-bonding interactions that propagates across the molecule, culminating in the ordering of switch II and placement of Q61 in the active site in a previously unobserved conformation. This structure suggests a direct catalytic role for Q61 where it interacts with a water molecule that bridges one of the gamma-phosphate oxygen atoms to the hydroxyl group of Y32 to stabilize the transition state of the hydrolysis reaction. We propose that Raf together with the binding of Ca(2+) and a negatively charged group mimicked in our structure by the acetate molecule induces the ordering of switch I and switch II to complete the active site of Ras.

PubMed: 20194776
DOI: 10.1073/pnas.0912226107

実験手法

X-RAY DIFFRACTION (1.862 Å)