3L9U

Crystal Structure of Salmonella enterica serovar Typhimurium DsbL

3L9U の概要

• エントリーDOI: 10.2210/pdb3l9u/pdb
• 関連するPDBエントリー: 1DSB 1FVK 3A3T 3BCI 3C7M 3F4R 3L9S 3L9V
• 分子名称: Disulfide isomerase (2 entities in total)
• 機能のキーワード: thioredoxin-fold, dsbl, thiol-disulfide oxidoreductase, isomerase, oxidoreductase
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22226.44

構造登録者

Heras, B.,Jarrott, R.,Shouldice, S.R.,Guncar, G. (登録日: 2010-01-05, 公開日: 2010-03-09, 最終更新日: 2024-10-09)

主引用文献

Heras, B.,Totsika, M.,Jarrott, R.,Shouldice, S.R.,Guncar, G.,Achard, M.E.S.,Wells, T.J.,Argente, M.P.,McEwan, A.G.,Schembri, M.A.
Structural and functional characterization of three DsbA paralogues from Salmonella enterica serovar typhimurium
J.Biol.Chem., 285:18423-18432, 2010

PubMed Abstract: In prototypic Escherichia coli K-12 the introduction of disulfide bonds into folding proteins is mediated by the Dsb family of enzymes, primarily through the actions of the highly oxidizing protein EcDsbA. Homologues of the Dsb catalysts are found in most bacteria. Interestingly, pathogens have developed distinct Dsb machineries that play a pivotal role in the biogenesis of virulence factors, hence contributing to their pathogenicity. Salmonella enterica serovar (sv.) Typhimurium encodes an extended number of sulfhydryl oxidases, namely SeDsbA, SeDsbL, and SeSrgA. Here we report a comprehensive analysis of the sv. Typhimurium thiol oxidative system through the structural and functional characterization of the three Salmonella DsbA paralogues. The three proteins share low sequence identity, which results in several unique three-dimensional characteristics, principally in areas involved in substrate binding and disulfide catalysis. Furthermore, the Salmonella DsbA-like proteins also have different redox properties. Whereas functional characterization revealed some degree of redundancy, the properties of SeDsbA, SeDsbL, and SeSrgA and their expression pattern in sv. Typhimurium indicate a diverse role for these enzymes in virulence.

PubMed: 20233716
DOI: 10.1074/jbc.M110.101360

実験手法

X-RAY DIFFRACTION (1.568 Å)