3L10

Structure of split monoubiquitinated PCNA with ubiquitin in position one

3L10 の概要

• エントリーDOI: 10.2210/pdb3l10/pdb
• 関連するPDBエントリー: 3L0W 3L0X
• 分子名称: Proliferating cell nuclear antigen, Monoubiquitinated Proliferating cell nuclear antigen (2 entities in total)
• 機能のキーワード: replication, dna damage, dna repair, dna replication, dna-binding, isopeptide bond, nucleus, ubl conjugation
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast); 詳細
• 細胞内の位置: Nucleus: P15873 P15873
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37953.30

構造登録者

Freudenthal, B.D.,Gakhar, L.,Ramaswamy, S.,Washington, M.T. (登録日: 2009-12-10, 公開日: 2010-03-23, 最終更新日: 2023-09-06)

主引用文献

Freudenthal, B.D.,Gakhar, L.,Ramaswamy, S.,Washington, M.T.
Structure of monoubiquitinated PCNA and implications for translesion synthesis and DNA polymerase exchange.
Nat.Struct.Mol.Biol., 17:479-484, 2010

PubMed Abstract: DNA synthesis by classical polymerases can be blocked by many lesions. These blocks are overcome by translesion synthesis, whereby the stalled classical, replicative polymerase is replaced by a nonclassical polymerase. In eukaryotes this polymerase exchange requires proliferating cell nuclear antigen (PCNA) monoubiquitination. To better understand the polymerase exchange, we developed a means of producing monoubiquitinated PCNA, by splitting the protein into two self-assembling polypeptides. We determined the X-ray crystal structure of monoubiquitinated PCNA and found that the ubiquitin moieties are located on the back face of PCNA and interact with it through their canonical hydrophobic surface. Moreover, the attachment of ubiquitin does not change PCNA's conformation. We propose that PCNA ubiquitination facilitates nonclassical polymerase recruitment to the back of PCNA by forming a new binding surface for nonclassical polymerases, consistent with a 'tool belt' model of the polymerase exchange.

PubMed: 20305653
DOI: 10.1038/nsmb.1776

実験手法

X-RAY DIFFRACTION (2.8 Å)